Journal ArticleDOI
Toward fluorescence nanoscopy
TLDR
A family of concepts has emerged that overcomes the diffraction barrier altogether and, relying on saturated optical transitions, these concepts are limited only by the attainable saturation level.Abstract:
For more than a century, the resolution of focusing light microscopy has been limited by diffraction to 180 nm in the focal plane and to 500 nm along the optic axis Recently, microscopes have been reported that provide three- to sevenfold improved axial resolution in live cells Moreover, a family of concepts has emerged that overcomes the diffraction barrier altogether Its first exponent, stimulated emission depletion microscopy, has so far displayed a resolution down to 28 nm Relying on saturated optical transitions, these concepts are limited only by the attainable saturation level As strong saturation should be feasible at low light intensities, nanoscale imaging with focused light may be closer than everread more
Citations
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Journal ArticleDOI
Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM).
TL;DR: A high-resolution fluorescence microscopy method based on high-accuracy localization of photoswitchable fluorophores that can, in principle, reach molecular-scale resolution is developed.
Journal ArticleDOI
Far-Field Optical Nanoscopy
TL;DR: Initial applications indicate that emergent far-field optical nanoscopy will have a strong impact in the life sciences and in other areas benefiting from nanoscale visualization.
Journal ArticleDOI
Three-Dimensional Super-Resolution Imaging by Stochastic Optical Reconstruction Microscopy
TL;DR: 3D stochastic optical reconstruction microscopy (STORM) is demonstrated by using optical astigmatism to determine both axial and lateral positions of individual fluorophores with nanometer accuracy, allowing the 3D morphology of nanoscopic cellular structures to be resolved.
Journal ArticleDOI
Subdiffraction-Resolution Fluorescence Imaging with Conventional Fluorescent Probes†
Mike Heilemann,Sebastian van de Linde,Mark Schüttpelz,Robert Kasper,Britta Seefeldt,Anindita Mukherjee,Philip Tinnefeld,Markus Sauer +7 more
Journal ArticleDOI
Multicolor Super-Resolution Imaging with Photo-Switchable Fluorescent Probes
TL;DR: A family of photo-switchable fluorescent probes is introduced and multicolor stochastic optical reconstruction microscopy (STORM) is demonstrated, to facilitate direct visualization of molecular interactions at the nanometer scale.
References
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Book
Principles of fluorescence spectroscopy
TL;DR: This book describes the fundamental aspects of fluorescence, the biochemical applications of this methodology, and the instrumentation used in fluorescence spectroscopy.
Book
Principles of Optics
TL;DR: In this paper, the authors discuss various topics about optics, such as geometrical theories, image forming instruments, and optics of metals and crystals, including interference, interferometers, and diffraction.
Principles of Optics
TL;DR: In this article, the authors discuss various topics about optics, such as geometrical theories, image forming instruments, and optics of metals and crystals, including interference, interferometers, and diffraction.
Journal ArticleDOI
Introduction to Fourier Optics
Joseph W. Goodman,Mary E. Cox +1 more
TL;DR: The second edition of this respected text considerably expands the original and reflects the tremendous advances made in the discipline since 1968 as discussed by the authors, with a special emphasis on applications to diffraction, imaging, optical data processing, and holography.
Book
Introduction to Fourier optics
TL;DR: The second edition of this respected text considerably expands the original and reflects the tremendous advances made in the discipline since 1968 as discussed by the authors, with a special emphasis on applications to diffraction, imaging, optical data processing, and holography.
Related Papers (5)
Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy
Stefan W. Hell,Jan Wichmann +1 more