Trapping of DNA in Nonuniform Oscillating Electric Fields
TLDR
DNA molecules can be manipulated in aqueous solution in a manner analogous to optical trapping by mixing static and oscillating electric fields, which should be useful in microdevices for manipulation of small quantities or single molecules of DNA.About:
This article is published in Biophysical Journal.The article was published on 1998-02-01 and is currently open access. It has received 226 citations till now. The article focuses on the topics: Electric field & Dipole.read more
Citations
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Transport phenomena in nanofluidics
TL;DR: In this paper, the authors investigated the transport properties of 50-nm-high 1D nanochannels on a chip and showed that they can be used for the separation and preconcentration of proteins.
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Review Article—Dielectrophoresis: Status of the theory, technology, and applications
TL;DR: Current trends suggest that the theory and technology have matured sufficiently for most effort to now be directed towards applying DEP to unmet needs in such areas as biosensors, cell therapeutics, drug discovery, medical diagnostics, microfluidics, nanoassembly, and particle filtration.
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Noise-based switches and amplifiers for gene expression.
TL;DR: A model describing the regulation of gene expression and the effects of noise are developed and it is suggested that an external noise source could be used as a switch and/or amplifier for gene expression.
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Separation of Submicron Bioparticles by Dielectrophoresis
TL;DR: With electrode arrays fabricated using direct write electron beam lithography, it is shown that different types of submicron latex spheres can be spatially separated and it is demonstrated that tobacco mosaic virus and herpes simplex virus can be manipulated and spatially separation in a microelectrode array.
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Electrodeless dielectrophoresis of single- and double-stranded DNA.
Chia-Fu Chou,Jonas O. Tegenfeldt,Olgica Bakajin,Olgica Bakajin,Shirley S. Chan,Edward C. Cox,N. Darnton,Thomas Duke,Robert H. Austin +8 more
TL;DR: This paper demonstrates dielectrophoretic trapping using insulating constrictions at far lower frequencies than are feasible with metallic trapping structures because of water electrolysis, and demonstrates that electrodelessdielectrophoresis (EDEP) can be used for concentration and patterning of both single-strand and double-stranded DNA.
References
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Forces of a single-beam gradient laser trap on a dielectric sphere in the ray optics regime.
TL;DR: It is shown that good trapping requires high convergence beams from a high numerical aperture objective and a comparison is given of traps made using bright field or differential interference contrast optics and phase contrast optics.
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A DNA microarray system for analyzing complex DNA samples using two-color fluorescent probe hybridization.
TL;DR: This work describes a general experimental approach, using microscopic arrays of DNA fragments on glass substrates for differential hybridization analysis of fluorescently labeled DNA samples, and demonstrates the utility of DNA microarrays in the analysis of complex DNA samples.
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Functional integration of PCR amplification and capillary electrophoresis in a microfabricated DNA analysis device.
Adam T. Woolley,Dean R. Hadley,Phoebe Landre,Andrew J. deMello,Richard A. Mathies,Northrup Ma +5 more
TL;DR: The feasibility of performing high-speed DNA analyses in microfabricated integrated fluidic systems is established, demonstrating that challenging amplifications of diagnostically interesting targets can also be performed.
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Ultra-high-speed DNA fragment separations using microfabricated capillary array electrophoresis chips.
TL;DR: Methods for high-speed, high-throughput DNA separations on capillary array electrophoresis chips are established and high-resolution electrophoretic separations of phi X174 Hae III DNA restriction fragments are performed using a hydroxyethyl cellulose sieving matrix in the channels.