Ubiquity and diversity of ammonia-oxidizing archaea in water columns and sediments of the ocean
Christopher A. Francis,Kathryn J. Roberts,J. Michael Beman,Alyson E. Santoro,Brian B. Oakley +4 more
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TLDR
Using PCR primers designed to specifically target archaeal amoA, AOA is found to be pervasive in areas of the ocean that are critical for the global nitrogen cycle, including the base of the euphotic zone, suboxic water columns, and estuarine and coastal sediments.Abstract:
Nitrification, the microbial oxidation of ammonia to nitrite and nitrate, occurs in a wide variety of environments and plays a central role in the global nitrogen cycle. Catalyzed by the enzyme ammonia monooxygenase, the ability to oxidize ammonia was previously thought to be restricted to a few groups within the β- and γ-Proteobacteria. However, recent metagenomic studies have revealed the existence of unique ammonia monooxygenase α-subunit (amoA) genes derived from uncultivated, nonextremophilic Crenarchaeota. Here, we report molecular evidence for the widespread presence of ammonia-oxidizing archaea (AOA) in marine water columns and sediments. Using PCR primers designed to specifically target archaeal amoA, we find AOA to be pervasive in areas of the ocean that are critical for the global nitrogen cycle, including the base of the euphotic zone, suboxic water columns, and estuarine and coastal sediments. Diverse and distinct AOA communities are associated with each of these habitats, with little overlap between water columns and sediments. Within marine sediments, most AOA sequences are unique to individual sampling locations, whereas a small number of sequences are evidently cosmopolitan in distribution. Considering the abundance of nonextremophilic archaea in the ocean, our results suggest that AOA may play a significant, but previously unrecognized, role in the global nitrogen cycle.read more
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Spatial Interaction of Archaeal Ammonia-Oxidizers and Nitrite-Oxidizing Bacteria in an Unfertilized Grassland Soil.
Barbara Stempfhuber,Tim Richter-Heitmann,Kathleen M. Regan,Angelika Kölbl,Pia K. Wüst,Sven Marhan,Johannes Sikorski,Jörg Overmann,Michael W. Friedrich,Ellen Kandeler,Michael Schloter +10 more
TL;DR: A positive correlation between numerically dominant AOA and Nitrospira, and their co-occurrence at the same spatial scale in August and October, suggests that the nitrification process is predominantly performed by these groups and is restricted to a limited timeframe.
Journal ArticleDOI
Time-dependent shifts in populations and activity of bacterial and archaeal ammonia oxidizers in response to liming in acidic soils
TL;DR: In this article, the authors investigated population dynamics and activity of ammonia oxidizers in response to a 2-year liming field trial in acidic soils received longterm fertilization with chemical N fertilizers and short-term lime amendment in microcosm incubations.
Journal ArticleDOI
Comammox Nitrospira Species Dominate in an Efficient Partial Nitrification–Anammox Bioreactor for Treating Ammonium at Low Loadings
Yung Hsien Shao,Jer Horng Wu +1 more
TL;DR: In this paper, a bench-scale bioreactor with continuous stirring was operated for more than 1000 days with limited oxygen supply to achieve efficient nitrogen removal (70.1 ± 2.7%) at a low ammonium loading of 35.2 mg-N/L/day.
Journal ArticleDOI
Linking plant identity and interspecific competition to soil nitrogen cycling through ammonia oxidizer communities
TL;DR: Results indicate that plant species and interspecific competition regulate soil nitrogen cycling via a mechanism of other than alteration in the community structure of ammonia oxidizers as investigated by DNA based methods.
Journal ArticleDOI
Research trends and hotspots related to ammonia oxidation based on bibliometric analysis.
TL;DR: A bibliometric analysis based on 4314 articles extracted from Science Citation Index Expanded database was carried out to provide insights into publication performances and research trends of ammonia oxidation in the period 1991–2014 and suggested that microbial abundance and community of AOA and AOB was the most prominent hotspot.
References
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