Journal ArticleDOI
Ultralow Fouling and Functionalizable Surface Chemistry Based on a Zwitterionic Polymer Enabling Sensitive and Specific Protein Detection in Undiluted Blood Plasma
Hana Vaisocherová,Wei Yang,Zheng Zhang,Zhiqiang Cao,Gang Cheng,Marek Piliarik,Jiří Homola,Shaoyi Jiang +7 more
TLDR
A zwitterionic poly(carboxybetaine acrylamide) (polyCBAA) biomimetic material was employed to create a unique biorecognition coating with an ultralow fouling background, enabling the sensitive and specific detection of proteins in blood plasma.Abstract:
A crucial step in the development of implanted medical devices, in vivo diagnostics, and microarrays is the effective prevention of nonspecific protein adsorption from real-world complex media such as blood plasma or serum. In this work, a zwitterionic poly(carboxybetaine acrylamide) (polyCBAA) biomimetic material was employed to create a unique biorecognition coating with an ultralow fouling background, enabling the sensitive and specific detection of proteins in blood plasma. Conditions for surface activation, protein immobilization, and surface deactivation of the carboxylate groups in the polyCBAA coating were determined. An antibody-functionalized polyCBAA surface platform was used to detect a target protein in blood plasma using a sensitive surface plasmon resonance (SPR) sensor. A selective protein was directly detected from 100% human blood plasma with extraordinary specificity and sensitivity. The total nonspecific protein adsorption on the functionalized polyCBAA surface was very low (<3 ng/cm2 ...read more
Citations
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Journal ArticleDOI
Ultralow-Fouling, Functionalizable, and Hydrolyzable Zwitterionic Materials and Their Derivatives for Biological Applications
Shaoyi Jiang,Zhiqiang Cao +1 more
TL;DR: Mixed-charge materials have been shown to be equivalent to zwitterionic materials in resisting nonspecific protein adsorption when they are uniformly mixed at the molecular scale.
Journal ArticleDOI
Targeted polymeric therapeutic nanoparticles: design, development and clinical translation
TL;DR: In this critical review, insights are provided into the design and development of targeted polymeric NPs and the challenges associated with the engineering of this novel class of therapeutics are highlighted, including considerations of NP design optimization, development and biophysicochemical properties.
Journal ArticleDOI
Surface Hydration: Principles and Applications Toward Low-fouling/nonfouling Biomaterials
TL;DR: In this article, two major classes of non-fouling materials (i.e., hydrophilic and zwitterionic materials) and associated basic non fouling mechanisms and practical examples are discussed.
Journal ArticleDOI
Nanoparticle Probes for the Detection of Cancer Biomarkers, Cells, and Tissues by Fluorescence
Alyssa B. Chinen,Chenxia M. Guan,Jennifer R. Ferrer,Stacey N. Barnaby,Timothy J. Merkel,Chad A. Mirkin +5 more
TL;DR: Overcoming Limitations in Nanoparticle Drug Delivery: Triggered, Intravascular Release to Improve Drug Penetration into Tumors and Design Considerations for Tumour-Targeted Nanoparticles.
Journal ArticleDOI
Molecular Understanding and Design of Zwitterionic Materials
Qing Shao,Shaoyi Jiang +1 more
TL;DR: A molecular design of new protein-resistant zwitterionic moieties beyond conventional CB and SB based on design principles developed from these simulation studies are demonstrated.
References
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Journal ArticleDOI
Surface Plasmon Resonance Sensors for Detection of Chemical and Biological Species
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A Survey of Structure−Property Relationships of Surfaces that Resist the Adsorption of Protein
TL;DR: In this paper, the authors used surface plasmon resonance spectroscopy and self-assembled monolayers (SAMs) to determine the characteristics of functional groups that give surfaces the ability to resist the nonspecific adsorption of proteins from solution.
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Surface plasmon resonance based sensors
TL;DR: Applications of SPR Sensors for Detection of Biological and Chemical Analytes and Investigating Biomolecular Interactions and Binding Properties Using SPR Biosensors.
Journal ArticleDOI
Zwitterionic SAMs that Resist Nonspecific Adsorption of Protein from Aqueous Buffer
TL;DR: This paper describes the use of surface plasmon resonance spectroscopy and self-assembled monolayers (SAMs) of alkanethiols on gold to evaluate the ability of surfaces terminating in different combinations of charged groups to resist the nonspecific adsorption of proteins from aqueous buffer.
Journal ArticleDOI
Zwitterionic polymers exhibiting high resistance to nonspecific protein adsorption from human serum and plasma
TL;DR: The zwitterionic polymer surface based on carboxybetaine methacrylate (CBMA) also incorporates functional groups for protein immobilization in the nonfouling background, making it a strong candidate for many applications such as in diagnostics and drug delivery.
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Ultralow-Fouling, Functionalizable, and Hydrolyzable Zwitterionic Materials and Their Derivatives for Biological Applications
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