Ultrastructural localization of capsules, cell wall polysaccharide, cell wall proteins, and F antigen in pneumococci.
TLDR
During the course of the experimental work a reproducible method of gold labeling immunoglobulins was developed and an extra layer on the cell walls was conspicuous on electron micrographs of both rough and encapsulated pneumococci.Abstract:
The localization of pneumococcal capsular and cell wall antigens was examined by immunoelectron microscopy. C polysaccharide (C-Ps), a common component of all pneumococci, was uniformly distributed on both the inside and outside of the cell walls. The thickness of the C-Ps varied with the strain. Encapsulated strains were covered by varied amounts of capsular polysaccharide concealing the C-Ps of the bacteria so as to render it inaccessible to anti-C-Ps antibodies. In addition to C-Ps, protein antigens were demonstrable on the surface of nonencapsulated pneumococci. The proteins were not masked by the C-Ps layer. An extra layer on the cell walls was conspicuous on electron micrographs of both rough and encapsulated pneumococci. The nature of this extra layer has not been disclosed. F antigen, another common antigen of pneumococci, was uniformly distributed on the surface of the plasma membranes. During the course of the experimental work a reproducible method of gold labeling immunoglobulins was developed.read more
Citations
More filters
Journal ArticleDOI
The role of Streptococcus pneumoniae virulence factors in host respiratory colonization and disease.
TL;DR: This Review describes how S. pneumoniae uses its armamentarium of virulence factors to colonize the upper and lower respiratory tracts of the host and cause disease.
Journal ArticleDOI
Streptococcus pneumoniae: virulence factors, pathogenesis, and vaccines.
TL;DR: Although pneumococcal conjugate vaccines are close to being licensed, a more profound knowledge of the virulence factors responsible for the morbidity and mortality caused by Streptococcus pneumoniae is necessary.
Journal ArticleDOI
Association of Intrastrain Phase Variation in Quantity of Capsular Polysaccharide and Teichoic Acid with the Virulence of Streptococcus pneumoniae
Jean O. Kim,Jeffrey N. Weiser +1 more
TL;DR: Results suggest that the pneumococcus phase varies between a virulence form with more capsular polysaccharide and less teichoic acid and an avirulent form with less capsularpolysaccharides and more te Jerichoic acid.
Journal ArticleDOI
CD4+ T cells mediate antibody-independent acquired immunity to pneumococcal colonization
Richard Malley,Krzysztof Trzciński,Amit Kumar Srivastava,Claudette M. Thompson,Porter W. Anderson,Marc Lipsitch +5 more
TL;DR: It is found that immunity to pneumococcal colonization can be induced in the absence of antibody, independent of the capsular type, and this protection requires the presence of CD4(+) T cells at the time of challenge.
Journal ArticleDOI
Description of 14 new capsular types of Streptococcus suis.
TL;DR: Fourteen new capsular types of Streptococcus suis are described and several cross-reactions probably not due to capsular material were detected among different types by using the coagglutination test.
References
More filters
Journal ArticleDOI
Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions
TL;DR: In this article, a series of monodisperse suspensions of the same chemical composition but of rather different particle sizes was used to study particle size dependent phenomena, such as Brownian motion, light scattering, sedimentation and electrophoresis of small particles.
Journal ArticleDOI
Immunization, isolation of immunoglobulins, estimation of antibody titre.
N. M. G. Harboe,Agnete Ingild +1 more
TL;DR: High titred monospecific antibodies are obtained in rabbits injecting as little as 25 μg pure antigen per kg body weight, and antibody titres as measured by 4 different titration methods are compared using anti‐human IgG from rabbits as a model.
Journal ArticleDOI
Preparation of adsorbents for biospecific affinity chromatography. Attachment of group-containing ligands to insoluble polymers by means of bifuctional oxiranes.
Lars Sundberg,Jerker Porath +1 more
TL;DR: In this article, a new method for preparing adsorbents for biospecific affinity chromatography is described, and the optimal conditions for the activation and subsequent coupling of proteins, peptides and aliphatic and aromatic amines have been evaluated.
Journal ArticleDOI
The Induction of Meningeal Inflammation by Components of the Pneumococcal Cell Wall
TL;DR: Generation of free cell wall components in cerebrospinal fluid as, for example, during treatment with antibiotics that are bacteriolytic as well as bactericidal, could contribute to increased inflammation in the subarachnoid space.
Book ChapterDOI
Chapter XI Laboratory Diagnosis, Serology and Epidemiology of Streptococcus pneumoniae
Erna Lund,Jørgen Henrichsen +1 more
TL;DR: If an isolated strain of gram-positive diplococci grows with typical colonies on blood agar and diffusely in serum broth, the final diagnosis of S. pneumonia becomes a matter of differentiating between this and other species of α-haemolytic streptococci.
Related Papers (5)
Association of Intrastrain Phase Variation in Quantity of Capsular Polysaccharide and Teichoic Acid with the Virulence of Streptococcus pneumoniae
Jean O. Kim,Jeffrey N. Weiser +1 more