Journal ArticleDOI
Urine molecular profiling distinguishes health and disease: new methods in diagnostics? Focus on UPLC–MS
Reads0
Chats0
TLDR
The combination of high-end analytical technology with multivariate statistics allows differential analysis of replicate samples with applications for both high- and low-molecular-weight analytes, namely proteins and metabolites.Abstract:
Urine may be a waste product, but it contains an enormous amount of information. Its role in diagnostics cannot be underestimated. The combination of high-end analytical technology with multivariate statistics allows differential analysis of replicate samples with applications for both high- and low-molecular-weight analytes, namely proteins and metabolites. Global urine profiles measured with NMR or mass spectrometry (MS)-based methods distinguish, for example, individuals, health status and hormonal changes. They do not necessarily discriminate between substance classes but investigate method-determined subgroups, such as all compounds separated by reversed-phase liquid chromatography. For diagnostics, the identification of those compounds is of secondary interest because the overall features of the profile itself are used for sample comparison. The potential of this simple approach for clinical diagnostics is huge, since only minimal urine preparation (e.g., centrifugation and filtration for liquid chr...read more
Citations
More filters
Journal ArticleDOI
Future Perspectives of Chinese Medical Formulae: Chinmedomics as an Effector
Xijun Wang,Aihua Zhang,Hui Sun +2 more
TL;DR: The scientific study of chinmedomics is at an early stage and requires further scrutiny and validation, but the approach has major implications to improve the efficacy ofchinmediformulae.
Journal ArticleDOI
Mass spectrometry-based metabolomics: applications to biomarker and metabolic pathway research.
TL;DR: This review will highlight the importance and benefit with certain characteristic examples of MS-metabolomics for identifying metabolic pathways and metabolites that accurately screen for potential diagnostic biomarkers of diseases.
Journal ArticleDOI
Cord blood metabolomic profiling in intrauterine growth restriction.
Donata Favretto,Erich Cosmi,Eugenio Ragazzi,Silvia Visentin,Marianna Tucci,Paolo Fais,Giovanni Cecchetto,Vincenzo Zanardo,Guido Viel,Santo Davide Ferrara +9 more
TL;DR: Non-targeted LC-HRMS analysis of cord blood collected at birth allowed the identification of significant differences in relative abundances of essential amino acids between IUGR and AGA fetuses, emerging as a promising tool for studying metabolic alterations.
Journal ArticleDOI
CO-occurring exposure to perchlorate, nitrate and thiocyanate alters thyroid function in healthy pregnant women.
Megan K. Horton,Benjamin C. Blount,Liza Valentin-Blasini,Ronald J. Wapner,Robin M. Whyatt,Chris Gennings,Pam Factor-Litvak +6 more
TL;DR: Co-exposure to perchlorate, nitrate and thiocyanate may alter maternal thyroid function, specifically TSH, during pregnancy.
Journal ArticleDOI
Biomarkers Discovery through Multivariate Statistical Methods: A Review of Recently Developed Methods and Applications in Proteomics
TL;DR: The most recent applications of multivariate methods for the identification of biomarkers in proteomics are reviewed with particular regard to the statistical methods exploited.
References
More filters
Journal ArticleDOI
Absolute Quantification of Proteins by LCMSE A Virtue of Parallel ms Acquisition
TL;DR: A new method of absolute quantification of proteins is described, based on the discovery of an unexpected relationship between MS signal response and protein concentration, which is constant within a coefficient of variation of less than ±10%.
Journal ArticleDOI
Global metabolic profiling procedures for urine using UPLC-MS.
Elizabeth J. Want,Ian D. Wilson,Helen G. Gika,Georgios Theodoridis,Robert S. Plumb,John P. Shockcor,Elaine Holmes,Jeremy K. Nicholson +7 more
TL;DR: The use of UPLC–MS for metabolic profiling in this way is not faster than the conventional HPLC-based methods but, because of improved chromatographic performance, provides superior metabolome coverage.
Journal ArticleDOI
The development of the DIGE system: 2D fluorescence difference gel analysis technology.
TL;DR: Ettan DigE is the system of technologies that has been optimized to fully benefit from the advantages provided by 2D DIGE, and builds on this technique by adding a highly accurate quantitative dimension.
Journal ArticleDOI
Quantitative proteomic analysis by accurate mass retention time pairs.
Jeffrey C. Silva,Richard Denny,Craig A. Dorschel,Marc V. Gorenstein,Ignatius J. Kass,Guo-Zhong Li,Therese McKenna,Michael J. Nold,Keith Richardson,Phillip Young,Scott J. Geromanos +10 more
TL;DR: The principal focus of this paper will demonstrate the quantitative aspects of the methodology and continue with a discussion of the associated, complementary qualitative capabilities.
Related Papers (5)
Mass spectrometric based approaches in urine metabolomics and biomarker discovery
A review of strategies for untargeted urinary metabolomic analysis using gas chromatography–mass spectrometry
The Human Urine Metabolome
Souhaila Bouatra,Farid Aziat,Rupasri Mandal,An Chi Guo,Michael Wilson,Craig Knox,Trent C. Bjorndahl,Ramanarayan Krishnamurthy,Fozia Saleem,Philip B. Liu,Zerihun T. Dame,Jenna Poelzer,Jessica Huynh,Faizath S. Yallou,Nick Psychogios,Edison Dong,Ralf Bogumil,Cornelia Roehring,David S. Wishart,David S. Wishart +19 more