Open AccessJournal Article
Use of dengue NS1 antigen for early diagnosis of dengue virus infection.
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TLDR
The results reveal that the dengue NS1 antigen test can be used to complement the current antibody test used in peripheral laboratories and could increase the diagnostic efficiency for early diagnosis of d Dengue virus infection.Abstract:
Accurate and timely diagnosis of dengue virus is important for early detection of dengue virus infection. In this study, the usefulness of the dengue NS1 antigen test was evaluated as a routine, rapid diagnostic test for dengue virus infection. A total of 208 sera from patients suspected of having dengue virus infection were collected and tested for dengue antibody, dengue genome and dengue NS1 antigen. Dengue antibody test, dengue PCR test and dengue antigen test were able to detect dengue virus infection from Days 1 to 8 in 72.8, 52.8 and 44.0% of samples, respectively. Of the 208 sera tested, 69.2% (144/208) of the acute sera were positive for dengue virus infection based on IgM antibody, IgG antibody, NS1 antigen and PCR tests. Thirty-two point two percent of the samples (67/208) were found positive for dengue NS1 antigen, 38.5% (80/208) were PCR positive, 40.9% (85/208) were IgM positive and 36.1% (75/208) were IgG positive for dengue virus. The results reveal the detection rate of dengue virus infection was similar for PCR and dengue antibody (65.9%) and for NS1 antigen and dengue antibody (62.0%) combinations. Therefore, the dengue NS1 antigen test can be used to complement the current antibody test used in peripheral laboratories. Thus, the combination of the NS1 antigen and antibody tests could increase the diagnostic efficiency for early diagnosis of dengue infection.read more
Citations
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References
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TL;DR: The accumulated data demonstrated that dengue viruses can be accurately detected and typed from viremic human serum samples.
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Enzyme-Linked Immunosorbent Assay Specific to Dengue Virus Type 1 Nonstructural Protein NS1 Reveals Circulation of the Antigen in the Blood during the Acute Phase of Disease in Patients Experiencing Primary or Secondary Infections
Sophie Alcon,Antoine Talarmin,Monique Debruyne,Andrew K. I. Falconar,Vincent Deubel,Marie Flamand +5 more
TL;DR: Interestingly, NS1 concentrations did not differ significantly in serum specimens obtained from patients experiencing primary or secondary dengue virus infections, indicating that NS1 protein detection may allow early diagnosis of infection.
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An Antigen Capture Enzyme-Linked Immunosorbent Assay Reveals High Levels of the Dengue Virus Protein NS1 in the Sera of Infected Patients
TL;DR: The presence of high levels of secreted NS1 in the sera of patients experiencing secondary d Dengue virus infections, and in the context of an anamnestic antibody response, suggests that NS1 may contribute significantly to the formation of the circulating immune complexes that are suspected to play an important role in the pathogenesis of severe dengue disease.
Journal ArticleDOI
Dengue diagnosis, advances and challenges
María G. Guzmán,Gustavo Kourí +1 more
TL;DR: An appropriate rapid, early and accessible diagnostic method useful both for epidemiological surveillance and clinical diagnosis is still needed, and laboratory infrastructure, technical expertise and research capacity must be improved in endemic countries in order to positively influence dengue surveillance.
Journal ArticleDOI
Evaluation of an Enzyme Immunoassay for Detection of Dengue Virus NS1 Antigen in Human Serum
Philippe Dussart,Bhety Labeau,Gisèle Lagathu,Philippe Louis,Márcio Roberto Teixeira Nunes,Sueli Rodrigues,Cécile Storck-Herrmann,Raymond Césaire,Jacques Morvan,Marie Flamand,Laurence Baril +10 more
TL;DR: NS1 antigen detection with the Platelia Dengue NS1 Ag kit could be used for first-line testing for acute dengue virus infection in clinical diagnostic laboratories.
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