Use of DNA barcodes to identify flowering plants
Reads0
Chats0
TLDR
Comparison of the total plastid genomes of tobacco and deadly nightshade enhanced with trials on widely divergent angiosperm taxa suggest that the sequences in this pair of loci have the potential to discriminate among the largest number of plant species for barcoding purposes.Abstract:
Methods for identifying species by using short orthologous DNA sequences, known as “DNA barcodes,” have been proposed and initiated to facilitate biodiversity studies, identify juveniles, associate sexes, and enhance forensic analyses. The cytochrome c oxidase 1 sequence, which has been found to be widely applicable in animal barcoding, is not appropriate for most species of plants because of a much slower rate of cytochrome c oxidase 1 gene evolution in higher plants than in animals. We therefore propose the nuclear internal transcribed spacer region and the plastid trnH-psbA intergenic spacer as potentially usable DNA regions for applying barcoding to flowering plants. The internal transcribed spacer is the most commonly sequenced locus used in plant phylogenetic investigations at the species level and shows high levels of interspecific divergence. The trnH-psbA spacer, although short (≈450-bp), is the most variable plastid region in angiosperms and is easily amplified across a broad range of land plants. Comparison of the total plastid genomes of tobacco and deadly nightshade enhanced with trials on widely divergent angiosperm taxa, including closely related species in seven plant families and a group of species sampled from a local flora encompassing 50 plant families (for a total of 99 species, 80 genera, and 53 families), suggest that the sequences in this pair of loci have the potential to discriminate among the largest number of plant species for barcoding purposes.read more
Citations
More filters
Journal ArticleDOI
Morphological and molecular identification of economically important Tortricidae (Lepidoptera) on tropical and subtropical fruit in South Africa
TL;DR: The inclusion of both morphological and molecular keys provides a reliable means of identifying the tortricids of economic importance on tropical and subtropical fruit in South Africa.
Journal ArticleDOI
Genetic diversity and structure of wild populations of Carica papaya in Northern Mesoamerica inferred by nuclear microsatellites and chloroplast markers
TL;DR: Evidence indicates that pre-Pleistocene events or refugia did not play an important role in the genetic structuring of wild papaya, and suggests that C. papaya was dispersed throughout the lowland rain forests of Mexico (along the coastal plains and foothills of Sierras).
Journal ArticleDOI
Organization of chloroplast psbA-trnH intergenic spacer in dicotyledonous angiosperms of the family Umbelliferae.
Galina V. Degtjareva,Maria D. Logacheva,Tahir H. Samigullin,E.I. Terentieva,C.M. Valiejo-Roman +4 more
TL;DR: The features revealed in spacer organization allow it as phylogenetic marker, and indels seem to be more informative for analyses than nucleotide substitutions, however, high conservation among closely related taxa and occurrence of homoplastic inversions in the stem-loop structure limit its application as DNA barcode.
Journal Article
DNA barcoding the medicinal plants of the genus Paris
TL;DR: ITS2 can be used to correctly identify medicinal plants of Paris, and it will be a potential DNA barcode for identifying medicinal Plants of other taxa.
Dna barcoding as a means for identifying medicinal plants of pakistan
TL;DR: DNA barcoding is being used to identify several medicinal plants found in Pakistan and distinguished them from other similar species.
References
More filters
Book
PCR protocols : A guide to methods and applications
TL;DR: Basic Methodology: M.A. Innis and D.F. Frohman, RACE: Rapid Amplification of cDNA Ends, and RNA Processing: Apo-B.R. Kwok, Procedure to Minimuze PCR-Product Carry-Over.
Journal ArticleDOI
Biological identifications through DNA barcodes
TL;DR: It is established that the mitochondrial gene cytochrome c oxidase I (COI) can serve as the core of a global bioidentification system for animals and will provide a reliable, cost–effective and accessible solution to the current problem of species identification.
Journal ArticleDOI
Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator
TL;DR: The results add to the evidence that cryptic species are prevalent in tropical regions, a critical issue in efforts to document global species richness, and illustrate the value of DNA barcoding, especially when coupled with traditional taxonomic tools, in disclosing hidden diversity.
Journal ArticleDOI
The complete nucleotide sequence of the tobacco chloroplast genome: its gene organization and expression.
Kazuo Shinozaki,Masaru Ohme,Minoru Tanaka,Tatsuya Wakasugi,Nobuaki Hayashida,Tohru Matsubayashi,N. Zaita,J. Chunwongse,Junichi Obokata,Kazuko Yamaguchi-Shinozaki,Chikara Ohto,Keita Torazawa,B. Y. Meng,Mamoru Sugita,Hiroshi Deno,T. Kamogashira,Kyoji Yamada,Jun Kusuda,F. Takaiwa,Akira Kato,N. Tohdoh,Hiroaki Shimada,Masahiro Sugiura +22 more
TL;DR: Five sequences coding for proteins homologous to components of the respiratory‐chain NADH dehydrogenase from human mitochondria have been found and sequence and expression analyses indicate both prokaryotic and eukaryotic features of the chloroplast genes.
Journal ArticleDOI
Identification of Birds through DNA Barcodes
TL;DR: The finding of large COI sequence differences between, as compared to small differences within, species confirms the effectiveness of COI barcodes for the identification of bird species, and implies that a standard screening threshold of sequence difference could speed the discovery of new animal species.
Related Papers (5)
A DNA barcode for land plants.
Peter M. Hollingsworth,Laura L. Forrest,John L. Spouge,Mehrdad Hajibabaei,Sujeevan Ratnasingham,Michelle van der Bank,Mark W. Chase,Robyn S. Cowan,David L. Erickson,Aron J. Fazekas,Sean W. Graham,Karen E. James,Ki Joong Kim,W. John Kress,Harald Schneider,Jonathan van Alphen-Stahl,Spencer C. H. Barrett,Cássio van den Berg,Diego Bogarín,Kevin S. Burgess,Kevin S. Burgess,Kenneth M. Cameron,Kenneth M. Cameron,Mark A. Carine,Juliana Chacón,Alexandra Clark,James J. Clarkson,Ferozah Conrad,Dion S. Devey,Caroline S. Ford,Terry A. Hedderson,Michelle L. Hollingsworth,Brian C. Husband,Laura J. Kelly,Laura J. Kelly,Prasad Kesanakurti,Jung Sung Kim,Young-Dong Kim,Renaud Lahaye,Hae-Lim Lee,David G. Long,Santiago Madriñán,Olivier Maurin,Isabelle Meusnier,Steven G. Newmaster,Chong-Wook Park,Diana M. Percy,Gitte Petersen,James E. Richardson,Gerardo A. Salazar,Vincent Savolainen,Vincent Savolainen,Ole Seberg,Mike J. Wilkinson,Dong-Keun Yi,Damon P. Little +55 more