Use of DNA barcodes to identify flowering plants
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TLDR
Comparison of the total plastid genomes of tobacco and deadly nightshade enhanced with trials on widely divergent angiosperm taxa suggest that the sequences in this pair of loci have the potential to discriminate among the largest number of plant species for barcoding purposes.Abstract:
Methods for identifying species by using short orthologous DNA sequences, known as “DNA barcodes,” have been proposed and initiated to facilitate biodiversity studies, identify juveniles, associate sexes, and enhance forensic analyses. The cytochrome c oxidase 1 sequence, which has been found to be widely applicable in animal barcoding, is not appropriate for most species of plants because of a much slower rate of cytochrome c oxidase 1 gene evolution in higher plants than in animals. We therefore propose the nuclear internal transcribed spacer region and the plastid trnH-psbA intergenic spacer as potentially usable DNA regions for applying barcoding to flowering plants. The internal transcribed spacer is the most commonly sequenced locus used in plant phylogenetic investigations at the species level and shows high levels of interspecific divergence. The trnH-psbA spacer, although short (≈450-bp), is the most variable plastid region in angiosperms and is easily amplified across a broad range of land plants. Comparison of the total plastid genomes of tobacco and deadly nightshade enhanced with trials on widely divergent angiosperm taxa, including closely related species in seven plant families and a group of species sampled from a local flora encompassing 50 plant families (for a total of 99 species, 80 genera, and 53 families), suggest that the sequences in this pair of loci have the potential to discriminate among the largest number of plant species for barcoding purposes.read more
Citations
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Authentication of the 31 species of toxic and potent Chinese Materia Medica by light microscopy, Part 3: Two species of T/PCMM from flowers and their common adulterants.
TL;DR: It is demonstrated that flower T/PCMM can be identified and authenticated using a light microscope equipped with an oil immersion lens, and this same equipment can be easily used to characterize other herbal flower medicines.
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Services of DNA barcoding in different fields.
TL;DR: An overview of DNA barcoding and its practical applications is provided and the limitation, future prospective and main informative platforms for DNA barCoding have also been discussed.
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A morphological and molecular study in the Deschampsia cespitosa complex (Poaceae; Poeae; Airinae) in northern North America.
TL;DR: Overall, the evidence points to the existence of a single species of Deschampsia cespitosa, a closely related group with several sequences and haplotypes that are nearly identical.
Journal ArticleDOI
Plant DNA barcoding: A test using Macaronesian taxa of Tolpis (Asteraceae)
TL;DR: Two combinations of four plastid markers that have been recommended for DNA barcoding were tested for utility in a putatively recently-diversified group: the primarily Canarian clade within the genus Tolpis (Asteraceae).
Journal ArticleDOI
Studying Genetic Variability of Pomegranate (Punica granatum L.) Based on Chloroplast DNA and Barcode Genes
TL;DR: Analysis of DNA barcodes in pomegranate suggested that two regions, psbE–petL and trnH–psbA, were more suitable for determining intra-species relationships of pome Granatum L. genotypes.
References
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Book
PCR protocols : A guide to methods and applications
TL;DR: Basic Methodology: M.A. Innis and D.F. Frohman, RACE: Rapid Amplification of cDNA Ends, and RNA Processing: Apo-B.R. Kwok, Procedure to Minimuze PCR-Product Carry-Over.
Journal ArticleDOI
Biological identifications through DNA barcodes
TL;DR: It is established that the mitochondrial gene cytochrome c oxidase I (COI) can serve as the core of a global bioidentification system for animals and will provide a reliable, cost–effective and accessible solution to the current problem of species identification.
Journal ArticleDOI
Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator
TL;DR: The results add to the evidence that cryptic species are prevalent in tropical regions, a critical issue in efforts to document global species richness, and illustrate the value of DNA barcoding, especially when coupled with traditional taxonomic tools, in disclosing hidden diversity.
Journal ArticleDOI
The complete nucleotide sequence of the tobacco chloroplast genome: its gene organization and expression.
Kazuo Shinozaki,Masaru Ohme,Minoru Tanaka,Tatsuya Wakasugi,Nobuaki Hayashida,Tohru Matsubayashi,N. Zaita,J. Chunwongse,Junichi Obokata,Kazuko Yamaguchi-Shinozaki,Chikara Ohto,Keita Torazawa,B. Y. Meng,Mamoru Sugita,Hiroshi Deno,T. Kamogashira,Kyoji Yamada,Jun Kusuda,F. Takaiwa,Akira Kato,N. Tohdoh,Hiroaki Shimada,Masahiro Sugiura +22 more
TL;DR: Five sequences coding for proteins homologous to components of the respiratory‐chain NADH dehydrogenase from human mitochondria have been found and sequence and expression analyses indicate both prokaryotic and eukaryotic features of the chloroplast genes.
Journal ArticleDOI
Identification of Birds through DNA Barcodes
TL;DR: The finding of large COI sequence differences between, as compared to small differences within, species confirms the effectiveness of COI barcodes for the identification of bird species, and implies that a standard screening threshold of sequence difference could speed the discovery of new animal species.
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