Use of DNA barcodes to identify flowering plants
Reads0
Chats0
TLDR
Comparison of the total plastid genomes of tobacco and deadly nightshade enhanced with trials on widely divergent angiosperm taxa suggest that the sequences in this pair of loci have the potential to discriminate among the largest number of plant species for barcoding purposes.Abstract:
Methods for identifying species by using short orthologous DNA sequences, known as “DNA barcodes,” have been proposed and initiated to facilitate biodiversity studies, identify juveniles, associate sexes, and enhance forensic analyses. The cytochrome c oxidase 1 sequence, which has been found to be widely applicable in animal barcoding, is not appropriate for most species of plants because of a much slower rate of cytochrome c oxidase 1 gene evolution in higher plants than in animals. We therefore propose the nuclear internal transcribed spacer region and the plastid trnH-psbA intergenic spacer as potentially usable DNA regions for applying barcoding to flowering plants. The internal transcribed spacer is the most commonly sequenced locus used in plant phylogenetic investigations at the species level and shows high levels of interspecific divergence. The trnH-psbA spacer, although short (≈450-bp), is the most variable plastid region in angiosperms and is easily amplified across a broad range of land plants. Comparison of the total plastid genomes of tobacco and deadly nightshade enhanced with trials on widely divergent angiosperm taxa, including closely related species in seven plant families and a group of species sampled from a local flora encompassing 50 plant families (for a total of 99 species, 80 genera, and 53 families), suggest that the sequences in this pair of loci have the potential to discriminate among the largest number of plant species for barcoding purposes.read more
Citations
More filters
Journal ArticleDOI
Phylogenetic and morphometric analysis of Plantago section Coronopus (Plantaginaceae)
TL;DR: The dataset provides the best sampled phylogenetic hypothesis for P. coronopus to date, and reveals discordance between nuclear and plastid genealogies within P. sect.
Journal ArticleDOI
Quality control of herbal drugs and preparations: The methods of analysis, their relevance and applications
TL;DR: In this paper, a review of analytical methods likely fit for each purpose of detecting adulterants and contaminants in herbal products is presented. But the authors focus on the analytical goals and their complexity, and do not consider the limiting factors such as product naming, sampling, and sample preparation.
Journal ArticleDOI
Genetic diversity of sago palm (Metroxylon sagu) accessions based on plastid cpDNA matK gene as DNA barcoding
TL;DR: Results of the study showed that the cpDNA sequence associated with matK genes in the genome of sago palm showed differences among accessions, which showed sago palms accessions separated into two genotypes.
Journal ArticleDOI
The complete plastome of Panax stipuleanatus: Comparative and phylogenetic analyses of the genus Panax (Araliaceae)
TL;DR: The plastome of Panax stipuleanatus (Araliaceae) has been sequenced in this article to better understand the relationships between species and plastomes evolution within the genus Panax.
Journal ArticleDOI
Chloroplast DNA analysis of the invasive weed, Himalayan balsam (Impatiens glandulifera), in the British Isles.
TL;DR: Results show where to focus future surveys in the native range to find more compatible rust strains ofImpatiens glandulifera, suggesting that HB was introduced into the BI on at least two occasions.
References
More filters
Book
PCR protocols : A guide to methods and applications
TL;DR: Basic Methodology: M.A. Innis and D.F. Frohman, RACE: Rapid Amplification of cDNA Ends, and RNA Processing: Apo-B.R. Kwok, Procedure to Minimuze PCR-Product Carry-Over.
Journal ArticleDOI
Biological identifications through DNA barcodes
TL;DR: It is established that the mitochondrial gene cytochrome c oxidase I (COI) can serve as the core of a global bioidentification system for animals and will provide a reliable, cost–effective and accessible solution to the current problem of species identification.
Journal ArticleDOI
Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator
TL;DR: The results add to the evidence that cryptic species are prevalent in tropical regions, a critical issue in efforts to document global species richness, and illustrate the value of DNA barcoding, especially when coupled with traditional taxonomic tools, in disclosing hidden diversity.
Journal ArticleDOI
The complete nucleotide sequence of the tobacco chloroplast genome: its gene organization and expression.
Kazuo Shinozaki,Masaru Ohme,Minoru Tanaka,Tatsuya Wakasugi,Nobuaki Hayashida,Tohru Matsubayashi,N. Zaita,J. Chunwongse,Junichi Obokata,Kazuko Yamaguchi-Shinozaki,Chikara Ohto,Keita Torazawa,B. Y. Meng,Mamoru Sugita,Hiroshi Deno,T. Kamogashira,Kyoji Yamada,Jun Kusuda,F. Takaiwa,Akira Kato,N. Tohdoh,Hiroaki Shimada,Masahiro Sugiura +22 more
TL;DR: Five sequences coding for proteins homologous to components of the respiratory‐chain NADH dehydrogenase from human mitochondria have been found and sequence and expression analyses indicate both prokaryotic and eukaryotic features of the chloroplast genes.
Journal ArticleDOI
Identification of Birds through DNA Barcodes
TL;DR: The finding of large COI sequence differences between, as compared to small differences within, species confirms the effectiveness of COI barcodes for the identification of bird species, and implies that a standard screening threshold of sequence difference could speed the discovery of new animal species.
Related Papers (5)
A DNA barcode for land plants.
Peter M. Hollingsworth,Laura L. Forrest,John L. Spouge,Mehrdad Hajibabaei,Sujeevan Ratnasingham,Michelle van der Bank,Mark W. Chase,Robyn S. Cowan,David L. Erickson,Aron J. Fazekas,Sean W. Graham,Karen E. James,Ki Joong Kim,W. John Kress,Harald Schneider,Jonathan van Alphen-Stahl,Spencer C. H. Barrett,Cássio van den Berg,Diego Bogarín,Kevin S. Burgess,Kevin S. Burgess,Kenneth M. Cameron,Kenneth M. Cameron,Mark A. Carine,Juliana Chacón,Alexandra Clark,James J. Clarkson,Ferozah Conrad,Dion S. Devey,Caroline S. Ford,Terry A. Hedderson,Michelle L. Hollingsworth,Brian C. Husband,Laura J. Kelly,Laura J. Kelly,Prasad Kesanakurti,Jung Sung Kim,Young-Dong Kim,Renaud Lahaye,Hae-Lim Lee,David G. Long,Santiago Madriñán,Olivier Maurin,Isabelle Meusnier,Steven G. Newmaster,Chong-Wook Park,Diana M. Percy,Gitte Petersen,James E. Richardson,Gerardo A. Salazar,Vincent Savolainen,Vincent Savolainen,Ole Seberg,Mike J. Wilkinson,Dong-Keun Yi,Damon P. Little +55 more