Journal ArticleDOI
Use of flow cytometric immunophenotyping to refine the cytological diagnosis of canine lymphoma.
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TLDR
In dogs, FC can add a number of useful diagnostic features to the morphological evaluation of lymphoma including the evaluation of B or T cell lineage, antigen quantification and evaluation of aberrant patterns, the assessment of clonality, staging and the Evaluation of minimal residual disease.Abstract:
The diagnosis of canine lymphoma is a multi-step process involving clinical examination, diagnostic imaging, cytology, haematology, biochemical profiling, histopathology and ancillary techniques such as flow cytometry (FC). In human medicine, FC (in addition to cytology) is reported to increase the accuracy of diagnosis of most lymphoma sub-types. In dogs, FC can add a number of useful diagnostic features to the morphological evaluation of lymphoma including the evaluation of B or T cell lineage, antigen quantification and evaluation of aberrant patterns, the assessment of clonality, staging and the evaluation of minimal residual disease. In comparison to other immunophenotyping techniques, FC is rapid and easy to perform and 'gating' techniques can resolve mixed cell populations although the use of fresh samples is required and the appropriate equipment and its maintenance are quite expensive. The use of FC to refine cytological diagnosis could be further enhanced by the use of a multi-parametric approach and by the development of a wider panel of standardised canine-specific antibodies.read more
Citations
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Journal ArticleDOI
The dog as a possible animal model for human non-Hodgkin lymphoma: a review.
TL;DR: The state‐of‐the‐art of canine lymphoma epidemiology, pathobiology, diagnostic work‐up and therapy is summarized, and the links to the corresponding human disease are highlighted, providing evidence for the use of dog as an animal model of spontaneous disease.
Journal ArticleDOI
Canine lymphoma: a review.
TL;DR: Canine lymphoma (cL) is a common type of neoplasia in dogs with an estimated incidence rate of 20–100 cases per 100,000 dogs and is in many respects comparable to non-Hodgkin lymphoma in humans.
Journal ArticleDOI
The Development of a Recombinant scFv Monoclonal Antibody Targeting Canine CD20 for Use in Comparative Medicine
Saurabh Jain,Luca Aresu,Stefano Comazzi,Jianguo Shi,Erin G. Worrall,John Clayton,William H. Humphries,Sandra Hemmington,Paul A. Davis,Euan Murray,Euan Murray,Asmare A. Limeneh,Kathryn L. Ball,Eva Ruckova,Petr Müller,Borek Vojtesek,Robin Fåhraeus,David Argyle,Ted R. Hupp +18 more
TL;DR: Immunohistochemical staining of canine tissues indicates that the NCD1.2 binds to clinically derived canine cells including B-cells in peripheral blood and in different histotypes of B-cell lymphoma, while data identify a recombinant anti-CD20 scFv that might form a useful tool for evaluation in bioconjugate-directed anti- CD20 immunotherapies in comparative medicine.
Journal ArticleDOI
Man's best friend: what can pet dogs teach us about non-Hodgkin's lymphoma?
TL;DR: Resources that are currently available to study canine lymphoma, advantages to be gained by exploiting the genetic breed structure in dogs, and current and future challenges and opportunities to take full advantage of this resource for lymphoma studies are described.
Journal ArticleDOI
Lymphoma immunophenotype of dogs determined by immunohistochemistry, flow cytometry, and polymerase chain reaction for antigen receptor rearrangements.
L. Thalheim,Laurel E. Williams,Luke B. Borst,Jonathan E. Fogle,Steven E. Suter,Steven E. Suter +5 more
TL;DR: Flow cytometry is superior to PARR in correctly predicting immunophenotypes when evaluating lymph nodes from dogs already diagnosed with B- or T-cell LSA and if fresh samples are not available for FC, PARR is an acceptable assay for determination of immunophenotype given its high specificity.
References
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Journal ArticleDOI
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Withrow & Macewen's Small Animal Clinical Oncology
TL;DR: Why worry about cancer in companion animals?
Journal ArticleDOI
Marker Expression in Peripheral T-Cell Lymphoma: A Proposed Clinical-Pathologic Prognostic Score
Philip Went,Claudio Agostinelli,Andrea Gallamini,Pier Paolo Piccaluga,Stefano Ascani,Elena Sabattini,Francesco Bacci,Brunangelo Falini,Teresio Motta,Marco Paulli,Tullio Artusi,Milena Piccioli,Pier Luigi Zinzani,Stefano Pileri +13 more
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Localized and Disseminated Histiocytic Sarcoma of Dendritic Cell Origin in Dogs
Verena K. Affolter,Peter F Moore +1 more
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Utility of Fine-Needle Aspiration As a Diagnostic Technique in Lymphoma
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