Using cell membrane chromatography and HPLC-TOF/MS method for in vivo study of active components from roots of Aconitum carmichaeli.
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TLDR
Binding affinities decreased along with the process of deacylation, debenzoylation and demethylation, which may be related to the alleviation of toxicity in the procedure of herb processing or metabolism.About:
This article is published in Journal of Pharmaceutical Analysis.The article was published on 2011-05-01 and is currently open access. It has received 8 citations till now. The article focuses on the topics: High-performance liquid chromatography.read more
Citations
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Comprehensive two-dimensional HepG2/cell membrane chromatography/monolithic column/time-of-flight mass spectrometry system for screening anti-tumor components from herbal medicines.
TL;DR: The result demonstrated that the proposed comprehensive 2D HepG2/CMC/monolithic column/TOFMS system has the advantages of strong recognition and rapid analysis abilities for the total screening procedure, which will be selectable and practical in drug discovery from complex HM samples and can also be applied to other biochromatography models.
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Recent advances in cell membrane chromatography for traditional Chinese medicines analysis
Xiaofang Hou,Sicen Wang,Tao Zhang,Jing Ma,Jie Zhang,Yanmin Zhang,Wen Lu,Huaizhen He,Langchong He +8 more
TL;DR: Some of the latest progress and applications about CMC including instrument development, research on drug-receptor interactions, screening active components from TCMs, and quality control of TCMs are reviewed.
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Recent advances in bio-affinity chromatography for screening bioactive compounds from natural products.
TL;DR: Current reported BAC stationary phases for natural products screening were particularly summarized, with a particular emphasis on the reported bio‐affinity stationary phases and chromatographic supports as well as the applications of BAC coupled with GC–MS or LC–MS.
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Identification of a ligand for tumor necrosis factor receptor from Chinese herbs by combination of surface plasmon resonance biosensor and UPLC-MS.
Yan Cao,Ying-Hua Li,Diya Lv,Xiaofei Chen,Langdong Chen,Zhenyu Zhu,Yifeng Chai,Jun-Ping Zhang +7 more
TL;DR: It was found for the first time that PMG was a ligand for TNF receptor from herbal medicines, and the proposed SPR-based screening method may prove to be an effective solution to analyzing bioactive components of Chinese herbs and other complex drug systems.
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A method for screening active components from Chinese herbs by cell membrane chromatography-offline-high performance liquid chromatography/mass spectrometry and an online statistical tool for data processing.
Yan Cao,Shaozhan Wang,Ying-Hua Li,Xiaofei Chen,Langdong Chen,Dongyao Wang,Zhenyu Zhu,Yongfang Yuan,Diya Lv +8 more
TL;DR: An offline 2D CMC-HPLC analysis combined with the XCMS (various forms of chromatography coupled to mass spectrometry) Online statistical tool for data processing is established and will be applicable in identifying active components from Chinese herbs and practical in discovery of lead compounds derived from herbs.
References
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Aconitum in traditional Chinese medicine: a valuable drug or an unpredictable risk?
TL;DR: This case study serves as a case study to explore the multidisciplinary implications of botanicals used in TCM, with a stipulation for a maximum level of DDA content of Aconitum highly desirable in order to guarantee the clinical safety and its low toxicity in decoctions.
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Screening for the anti-inflammatory activity of fractions and compounds from Atractylodes macrocephala koidz.
TL;DR: The results indicated that the retention characteristics of the petrol-ether (1:1, v/v) fraction (BZC-2) of the supercritical CO(2) extract, the atractylenolide I and 14-acetoxy-12-senecioyloxytetradeca-2E,8E,10E-trien-4,6-diyn-1-ol were the main components of Atractylodes macroce
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Coating and fusing cell membranes onto a silica surface and their chromatographic characteristics
TL;DR: It is concluded that CMSP may be used not only as a kind of packing material in bio-affinity chromatography, but also as a tool for studying the interactions between a drug and its receptor.
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Screening, analysis and in vitro vasodilatation of effective components from Ligusticum Chuanxiong.
TL;DR: Results demonstrated that ligustilide and butylidenephthalide can act on rat artery cell membrane similar to verapamil in CMC system and significantly inhibited the vasoconstrictions induced by norepinephrine bitartrate and calcium chloride.
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Enzymatic activity and chromatographic characteristics of the cell membrane immobilized on silica surface
TL;DR: A new cell membrane preparation, silica carrier cell membrane (CCM), has been presented, in which cell membrane was immobilized on silica which acted as carrier by the absorption, which showed that the silica CCM still keeps the same enzymatic activity as the other two states of cell membranes.