Virus‐induced gene silencing in Solanum species
Gianinna Brigneti,Ana Montserrat Martín-Hernández,Hailing Jin,Hailing Jin,Judy Chen,Judy Chen,David C. Baulcombe,Barbara Baker,Barbara Baker,Jonathan D. G. Jones +9 more
TLDR
It is reported that VIGS using the tobacco rattle virus (TRV) viral vector can be used in several Solanum species, although the choice of vector and experimental conditions vary depending on the species under study.Abstract:
Virus-induced gene silencing (VIGS) has been used routinely in Nicotiana benthamiana to assess functions of candidate genes and as a way to discover new genes required for diverse pathways, especially disease resistance signalling. VIGS has recently been shown to work in Arabidopsis thaliana and in tomato. Here, we report that VIGS using the tobacco rattle virus (TRV) viral vector can be used in several Solanum species, although the choice of vector and experimental conditions vary depending on the species under study. We have successfully silenced the phytoene desaturase (PDS) gene in the diploid wild species Solanum bulbocastanum and S. okadae, in the cultivated tetraploid S. tuberosum and in the distant hexaploid relative S. nigrum (commonly known as deadly nightshade). To test whether the system could be utilised as a rapid way to assess gene function of candidate resistance (R) genes in potato and its wild relatives, we silenced R1 and Rx in S. tuberosum and RB in S. bulbocastanum. Silencing of R1, Rx and RB successfully attenuated R-gene-mediated disease resistance and resulted in susceptible phenotypes in detached leaf assays. Thus, the VIGS system is an effective method of rapidly assessing gene function in potato.read more
Citations
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Genetic dissection of Verticillium wilt resistance mediated by tomato Ve1
Emilie F. Fradin,Zhao Zhang,Juan C. Juarez Ayala,Christian Danve M. Castroverde,Ross N. Nazar,Jane Robb,Chun-Ming Liu,Bart P. H. J. Thomma +7 more
TL;DR: It is shown that Ve1, but not Ve2, provides resistance in tomato against race 1 strains of V. albo-atrum and not against race 2 strains, and Ve1-mediated resistance signaling only partially overlaps with signaling mediated by Cf proteins, type members of the receptor-like protein class of resistance proteins.
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Efficient Virus-Induced Gene Silencing in Arabidopsis
TL;DR: It is shown that VIGS can be used to silence genes involved in general metabolism and defense and it is also effective at knocking down expression of highly expressed transgenes.
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Virus-induced gene silencing in tomato fruit.
TL;DR: The results of this study indicate that the application of VIGS techniques by the described methods can be successfully applied to tomato fruit and is a valuable tool for studying functions of the relevant genes during fruit developing.
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Moving forward in reverse: genetic technologies to enable genome-wide phenomic screens in Arabidopsis
Jose M. Alonso,Joseph R. Ecker +1 more
TL;DR: The combination of classical forward genetics with recently developed genome-wide, gene-indexed mutant collections is beginning to revolutionize the way in which gene functions are studied in plants.
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Hemipterans as plant pathogens
TL;DR: Responses to known defense signals and characterization of the signaling pathways controlled by the first cloned insect R gene (Mi-1) indicate that perception and signal transduction leading to resistance may be similar to plant-pathogen interactions.
References
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Journal ArticleDOI
Virus-induced gene silencing in tomato.
TL;DR: It is shown that recombinant TRV infects tomato plants and induces efficient gene silencing, and a modified TRV vector based on the GATEWAY recombination system is constructed, allowing restriction- and ligation-free cloning of tomato ESTs.
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An RNA-Dependent RNA Polymerase Gene in Arabidopsis Is Required for Posttranscriptional Gene Silencing Mediated by a Transgene but Not by a Virus
TL;DR: It is proposed that the role of SDE1 is to synthesize a double-stranded RNA initiator of posttranscriptional gene silencing, according to this idea, when a virus induces posttranscriptal genesilencing, the virus-encoded RNA polymerase would produce the double-Stranded RNA and Sde1 would be redundant.
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Technical Advance: Tobacco rattle virus as a vector for analysis of gene function by silencing
TL;DR: An infectious cDNA clone of tobacco rattle virus that has been modified to facilitate insertion of non-viral sequence and subsequent infection to plants is described and it is shown that this vector mediates VIGS of endogenous genes in the absence of virus-induced symptoms.
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Tobacco Rar1, EDS1 and NPR1/NIM1 like genes are required for N‐mediated resistance to tobacco mosaic virus
TL;DR: EDS1-like gene requirement for the N function suggests that EDS1 could be a common component of bacterial, fungal and viral resistance signalling mediated by the TIR-NBS-LRR class of resistance proteins.
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The Rx Gene from Potato Controls Separate Virus Resistance and Cell Death Responses
TL;DR: It is shown in both Nicotiana spp and potato that Rx has the potential to initiate a cell death response but that extreme resistance is separate and epistatic to necrosis, indicating that cell death and pathogen arrest are separate disease resistance responses in plants.