Journal ArticleDOI
Zinc and energy requirements in induction of oxidative stress to retinal pigmented epithelial cells
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TLDR
It is concluded that a combination of zinc and antioxidants or energy substrates rather that zinc alone should provide a safer and more effective way to treat a disease such as AMD.Abstract:
In age-related macular degeneration (AMD), retinal pigmented epithelium (RPE) cells are believed to be detrimentally affected. It is thought that zinc may play a part in this process. In the past, therefore, zinc supplementation has been suggested as a treatment for AMD. Experimental data shown here confound this view by indicating that whereas low amounts of zinc do protect RPE cells in culture from stress-induced effects, greater amounts of zinc have the opposite influence. These effects are partly dependent upon the “health status” of the cells. Experimental data presented herein also show that zinc-induced death of RPE cells can, however, be attenuated by compounds such as antioxidants (α-tocopherol, trolox, and metipranolol), or cellular energy substrates (pyruvate and oxaloacetate). It is therefore concluded that a combination of zinc and antioxidants or energy substrates rather that zinc alone should provide a safer and more effective way to treat a disease such as AMD.read more
Citations
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Journal ArticleDOI
The Zinc-Metallothionein Redox System Reduces Oxidative Stress in Retinal Pigment Epithelial Cells
Sara Rodríguez-Menéndez,Montserrat García,Beatriz Fernández,Lydia Álvarez,Andrés Fernández-Vega-Cueto,Miguel Coca-Prados,Rosario Pereiro,Héctor González-Iglesias +7 more
TL;DR: It is concluded that the stoichiometry of Zn-MT plays an important role in oxidative stress response, related with cellular metal homeostasis.
Journal ArticleDOI
Increased metallothionein in light damaged mouse retinas
TL;DR: Increased expression of the antioxidant MT in the light damaged mouse retina suggests that upregulation of MT is an important acute retinal response to photo-oxidative stress.
Journal ArticleDOI
Light-induced retinal degeneration is prevented by zinc, a component in the age-related eye disease study formulation.
Daniel T. Organisciak,Paul Wong,Christine M. Rapp,Ruth M. Darrow,Alison Ziesel,Rekha Rangarajan,John C. Lang +6 more
TL;DR: Although these data suggest an integrated and extensive regulatory response, zinc induced changes in gene expression also appear to enhance antioxidative capacity in retina and reduce oxidative damage arising from intense light exposure.
Journal ArticleDOI
The β-Adrenergic Receptor Antagonist Metipranolol Blunts Zinc-Induced Photoreceptor and RPE Apoptosis
TL;DR: The combined data suggest that oxidative injury to RPE cells and photoreceptors may be caused by elevated levels of zinc in diseases such as age-related macular degeneration (AMD) and that metipranolol may act as an efficacious antioxidant to blunt this process.
Journal ArticleDOI
Mitochondrial inhibition in rat retinal cell cultures as a model of metabolic compromise: mechanisms of injury and neuroprotection.
TL;DR: Mitochondrial respiratory inhibition via NaN(3) treatment, with delineated mechanisms of toxicity and neuroprotection, represents a valid and reproducible metabolic challenge to cultured retinal cells.
References
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Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
TL;DR: A tetrazolium salt has been used to develop a quantitative colorimetric assay for mammalian cell survival and proliferation and is used to measure proliferative lymphokines, mitogen stimulations and complement-mediated lysis.
Journal ArticleDOI
A randomized, placebo-controlled, clinical trial of high-dose supplementation with vitamins C and E, beta carotene, and zinc for age-related macular degeneration and vision loss: AREDS report no. 8
A. Kassoff,J. Kassoff,J. Buehler,M. Eglow,F. Kaufman,M. Mehu,S. Kieval,M. Mairs,B. Graig,A. Quattrocchi,David R. Jones,J. Locatelli,Alan J. Ruby,A Jr Capon,Bruce R. Garretson,Tarek S Hassan,Michael T. Trese,George A. Williams,V. Regan,P. Manatrey,P. Streasick,L. Szydlowski,F. McIver,C. Bridges,C. Stanley,K. Cumming,B. Lewis,Mary Zajechowski,R. R. Margherio,M. S. Cox,J. C. Werner,R. Falk,P. Siedlak,C. Neubert,Michael L. Klein,J. T. Stout,A. O'Malley,Andreas K. Lauer,Joseph E. Robertson,David J. Wilson,C. Beardsley,H. Anderson,P. Wallace,G. Smith,Shannon Howard,Richard F. Dreyer,C. Ma,R. G. Chenoweth,John Zilis,M. Johnson,Patrick B. Rice,H. Daniel,H. Crider,Stuart G Parker,K. Sherman,Daniel F. Martin,T M Sr Aaberg,P Jr Sternberg,Linda Curtis,B. Ju,James Gilman,B. Myles,S. Strittman,Christina Gentry,H. Yi,A Jr Capone,M. Lambert,Travis A. Meredith,T M Jr Aaberg,D. Saperstein,J. I. Lim,B. Stribling,D. Armiger,R. Swords,David H. Orth,T. P. Flood,J. Civantos,S. deBustros,Kirk H. Packo,Pauline T. Merrill,J. A. Cohen,Celeste Figliulo,Chris Morrison,D. A. Bryant,Don Doherty,M. McVicker,T. Drefcinski,J. M. Seddon,M. K. Pinnolis,N. Davis,I. Burton,T. Taitsel,David I. Walsh,J. Dubois-Moran,C. Callahan,Claudia Evans,K. K. Snow,D. A. Jones-Devonish,V. D. Crouse +98 more
TL;DR: People older than 55 years should have dilated eye examinations to determine their risk of developing advanced AMD and those with extensive intermediate size drusen, at least 1 large druse, noncentral geographic atrophy in 1 or both eyes, or advanced AMD or vision loss due to AMD in 1 eye should consider taking a supplement of antioxidants plus zinc.
Journal ArticleDOI
Dietary Carotenoids, Vitamins A, C, and E, and Advanced Age-Related Macular Degeneration
Johanna M. Seddon,Umed A. Ajani,Robert D. Sperduto,Rita Hiller,Norman P. Blair,Thomas C. Burton,Marilyn D. Farber,Evangelos S. Gragoudas,Julia A. Haller,Dayton T. Miller,Lawrence A. Yannuzzi,Walter C. Willett +11 more
TL;DR: Increasing the consumption of foods rich in certain carotenoids, in particular dark green, leafy vegetables, may decrease the risk of developing advanced or exudative AMD, the most visually disabling form of macular degeneration among older people.
Journal ArticleDOI
The proton-linked monocarboxylate transporter (MCT) family: structure, function and regulation
TL;DR: There is still much work to be done to characterize the properties of the different MCT isoforms and their regulation, which may have wide-ranging implications for health and disease.
Journal ArticleDOI
Zinc and brain injury
Dennis W. Choi,J.-Y. Koh +1 more
TL;DR: Manipulations aimed at reducing extracellular zinc accumulation, or cellular vulnerability to toxic zinc exposure, may provide a novel therapeutic approach toward ameliorating pathological neuronal death in these settings.
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