Showing papers on "Campesterol published in 1972"
TL;DR: During germination, sitosterol was the major sterol in all fractions but stigmasterol and campesterol showed the greatest changes, and the fatty acid composition of the steryl esters and acylated steryl glycosides most closely resembled the di- and triglycerides.
Abstract: The identity, composition, and concentration of the total, free, esterified, and glycosidic sterol fractions were determined during germination of tobacco seeds. The total, free, and esterified sterols increased, with stigmasterol and campesterol accounting for most of the increase. Steryl glycosides decreased during germination, and stigmasteryl and sitosteryl glycosides showed the largest decrease. During germination, sitosterol was the major sterol in all fractions but stigmasterol and campesterol showed the greatest changes. The fatty acid composition of the steryl esters and acylated steryl glycosides most closely resembled the di- and triglycerides.
92 citations
TL;DR: It is documented that a significant amount of cholesterol was excreted from the skin surface and that probably there was a net transfer of plasma cholesterol into the skinsurface lipids, which may need to be considered in sterol balance studies.
Abstract: The 24 hr sterol excretion from the entire skin surface was determined in six normal and five hypercholesterolemic (Type II) patients fed a controlled, eucaloric diet containing 400 mg of plant sterols. All subjects received radiolabeled cholesterol intravenously in order to measure cholesterol turnover and exchange. The 24 hr skin surface lipids were collected subsequently at intervals of 7-10 days. Sterols were quantified and identified by a combination of thin-layer and gas-liquid chromatographic methods. The mean 24 hr excretion of cholesterol in milligrams was 82.6 in the normal subjects and 82.7 in the hypercholesterolemic patients. Cholesterol constituted 89% of the total sterol excretion through the skin surface in both groups. The specific radioactivity of cholesterol in the skin surface lipids increased gradually after the intravenous administration of the isotope. Within 4-5 wk the specific activity equaled and then remained higher than that of the plasma up to 10 wk. These specific activity curves suggested that, for at least some of skin surface cholesterol, there was a precursor-product relationship between the plasma cholesterol and the skin cholesterol. The presence of plant sterols, beta-sitosterol, campesterol, and stigmasterol in the skin surface lipids of man has not been reported previously. We identified these sterols in the skin surface lipids of all of our subjects. They constituted about 7% of the total skin surface sterols. The occurrence of plant sterols in the skin surface lipids suggested that plasma sterols were transferred from the plasma into the skin. 1-2% of the skin surface sterols were tentatively identified as lathosterol and lanosterol. The present study documented that a significant amount of cholesterol was excreted from the skin surface and that probably there was a net transfer of plasma cholesterol into the skin surface lipids. Both normal subjects and hypercholesterolemic patients excreted similar amounts of cholesterol per day into the skin surface lipids. We suggest that this daily loss of cholesterol from the skin surface may need to be considered in sterol balance studies.
50 citations
TL;DR: The sterols are present in all subcellular fractions but their concentrations differ significantly; in particular the chloroplasts and the mitochondria are much richer in cholesterol compared to the leaves, and the role of the sterols in the structure and function of plant cell membranes is discussed.
49 citations
TL;DR: The major sterol fraction in all species was the Δ 5 sterols consisting mainly of cholesterol, campesterol, stigmasterol and β-sitosterol, which was the major plant sterol in all the species.
Abstract: 1. 1. Of the species examined, only chicken produced feces containing 5β-stanols (coprostanol derivatives). 2. 2. Feces of all species contained 5α-stanols; that of the White Carneau pigeon contained the most. 3. 3. The major sterol fraction in all species was the Δ 5 sterols consisting mainly of cholesterol, campesterol, stigmasterol and β-sitosterol. 4. 4. The proportions of these sterols were different in the different species. β-Sitosterol was the major plant sterol in all the species.
26 citations
TL;DR: The study undertaken shows the existence of water-soluble complexes of sterols, sterol esters and their precursors (triterpenes and 4α-methylsterols) in a higher plant, Zea mays L.
Abstract: The study undertaken shows the existence of water-soluble complexes of sterols, sterol esters and their precursors (triterpenes and 4α-methylsterols) in a higher plant, Zea mays L. The leaves, roots and culture medium were treated separately in order to investigate the role and nature of these complexes. The sterols found in the water-soluble form represent 0.1–1% by weight of the total sterols in the plant. The composition of the hydrosoluble fraction of the sterols and their precursors is different from that of the lipid-soluble fraction, cholesterol, sitosterol and isofucosterol being preferentially complexed compared to campesterol and stigmasterol.
This complex was found in the microsomal supernatant after homogenisation and also in the culture medium. The sterols are released from the complex by a treatment with pyrogallol under basic conditions or after repeated extraction with petroleum ether. The complex is precipitated with ethanol but not with trichloroacetic acid. The behaviour of several sterols on treatment with a solution of starch was investigated. Starch was able to complex with a large number of phytosterols unspecifically. The implication of this complex for the transport of sterols, for the biosynthesis of steroids and the ecological impact is discussed.
26 citations
TL;DR: 24-Methylenecholesterol was positively identified as an intermediate in the dealkylation and the conversion of campesterol to cholesterol in the tobacco hornworm, Manduca sexta.
Abstract: 24-Methylenecholesterol was positively identified as an intermediate in the dealkylation and the conversion of campesterol to cholesterol in the tobacco hornworm,Manduca sexta (L.). Biochemical and nutritional studies with3H-labeled campesterol and 24-methyl-enecholesterol indicated that the conversion of campesterol to cholesterol in this insect includes the following metabolic sequence: campesterol, 24-methylene-cholesterol, desmosterol, cholesterol.
25 citations
TL;DR: The level of stigmast-7-enol in infected leaves appeared to be correlated with the amount of fungal mass in the host, and the level of cholesterol tended to decrease after germination, especially in differentiating uredosporelings.
Abstract: Sterols extracted from healthy and rust-infected primary leaves of wheat or from non-germinated and germinated uredospores of stem rust were analyzed by thin-layer, column, and gas–liquid chromatography.Wheat leaves of susceptible and resistant lines contained cholesterol, campesterol, stigmasterol, β-sitosterol, and an unknown sterol that accounted for 75% to 80% of total sterol content. A further sterol, stigmast-7-enol, occurred in all rust-infected leaves and in 1 of 11 samples of healthy leaves. Sterol levels were not related to susceptibility or resistance. The level of stigmast-7-enol in infected leaves appeared to be correlated with the amount of fungal mass in the host.Uredospores of stem rust contained trace amounts of an unknown sterol, cholesterol, and either ergost-7-enol or stigmasterol, and larger amounts of stigmast-7-enol. After germination, the level of cholesterol increased, especially in differentiating uredosporelings. The level of stigmast-7-enol tended to decrease after germination....
23 citations
TL;DR: There was a general decrease in total sterol during the final maturation and curing phases of the tobacco plant and light does not appear to have a large effect on the distribution of campesterol and cholesterol.
23 citations
19 citations
TL;DR: The patterns of α-onocerin and sterols found seem to be consistent with the accepted classification of species within the genus Ononis, although in some others its concentration was low.
15 citations
TL;DR: Grapefruit seed oil sterols separated from other lipids by Florisil column chromatography were characterized by gas liquid chromatography as discussed by the authors, showing that stigmasterol, campesterol and β-sitosterol are present in proportions of 2.5, 7.4 and 90.1% of the total.
Abstract: Grapefruit seed oil sterols separated from other lipids by Florisil column chromatography were characterized by gas liquid chromatography. The presence of stigmasterol, campesterol and β-sitosterol is indicated. Expressed in terms of peak area, the three sterols are present in proportions of 2.5%, 7.4% and 90.1% of the total, respectively.
TL;DR: A new compound, nodolidate, has been isolated from the flowers of Cassia nodosa and characterized as (-)-7-acetoxy-9,10-dimethyl-1,5-octacosanolide.
TL;DR: In evaluating phylogenetic relationship between amphiploid species and the possible diploid progenitors, the results of sterol composition are in favor of N. tabacum as the hybrid combination from which N. undulata and N. paniculata were evolved.
Abstract: Stigmasterol, β-sitosterol, campesterol, and cholesterol are the predominant sterols identified by gasliquid chromatographic techniques in the mature leaves of 50 Nicotiana species. The relative composition pattern of the four sterols varies significantly among the subgenera as well as within the subgenus. However, six N. tabacum cultivars showed a similar pattern, of which as an average stigmasterol represents the highest proportion (43%) followed by β-sitosterol (30%), campesterol (19%), and cholesterol (8%) in total sterol content. Negative correlations were obtained for the composition of stigmasterol vs. β-sitosterol, cholesterol vs. campesterol, and cholesterol vs. β-sitosterol. Some correlations between geographic distribution of Nicotiana species and sterol composition were evident. In evaluating phylogenetic relationship between amphiploid species and the possible diploid progenitors, the results of sterol composition are in favor of N. undulata and N. paniculata being the ancestors of N. rustica and the N. sylvestris X N. tomentosiformis as the hybrid combination from which N. tabacum was evolved.
TL;DR: By means of column chromatography, TLC, GLC and GLC-mass spectrometry, the following sterols and triterpenes were found in free form in the pitcher plant, ( Nepenthes albomarginata): cholesterol, campesterol, stigmasterol, sitosterol, isofuctosterol and β-amyrin.
TL;DR: The method is accurate and reproducible and of great value in the study of cholesterol metabolism when the diet and feces contain a variety of plant sterols.