Showing papers on "Histone demethylation published in 2008"
TL;DR: It is reported that a Drosophila melanogaster JmjC domain-containing protein, dKDM4A, is a histone H3K36 demethylase, and in vivo evidence suggesting that heterochromatin protein 1a (HP1a) associates with dKDMA4A and that loss of HP1a leads to an increased level of hist one H 3K36me3.
96 citations
TL;DR: SMCX is a novel Smad3 corepressor that may antagonize the tumor suppressing activity of the TGF-beta/Smad3 signaling pathway and thereby contribute to tumorigenesis.
45 citations
01 Jan 2008
TL;DR: In this article, a proteomic analysis of mitotic chromosome scaffolds led to the identification of CENP-V, a novel kinetochore protein related to a bacterial enzyme that detoxifies formaldehyde, a byproduct of histone demethylation in eukaryotic cells.
Abstract: The mechanism of mitotic chromosome condensation is poorly understood, but even less is known about the mechanism of formation of the primary constriction, or centromere. A proteomic analysis of mitotic chromosome scaffolds led to the identification of CENP-V, a novel kinetochore protein related to a bacterial enzyme that detoxifies formaldehyde, a by-product of histone demethylation in eukaryotic cells. Overexpression of CENP-V leads to hypercondensation of pericentromeric heterochromatin, a phenotype that is abolished by mutations in the putative catalytic site. CENP-V depletion in HeLa cells leads to abnormal expansion of the primary constriction of mitotic chromosomes, mislocalization and destabilization of the chromosomal passenger complex (CPC) and alterations in the distribution of H3K9me3 in interphase nucleoplasm. CENP-V-depleted cells suffer defects in chromosome alignment in metaphase, lagging chromosomes in anaphase, failure of cytokinesis and rapid cell death. CENP-V provides a novel link between centromeric chromatin, the primary constriction and the CPC.
30 citations
TL;DR: An in-depth discussion on recent results and three important points were provided, which will be beneficial to a better understanding of demethylases and suggest how future research can be improved.
Abstract: The study of histone lysine demethylases has become very hot recently. Many histone demethylases have been reported by different research groups with various techniques. However, how many histone lysine-methylation states can be removed by one specific demethylase and how many demethylases can remove one specific histone lysine-methylation state? It remains a daunting challenge to answer these questions to date. An in-depth discussion on recent results, three important points were provided: (1) Some demethylases can remove more histone lysine-methylation states; (2) Some prokaryotes might be endowed with histone lysine demethylases although they are devoid of histones; (3) Protein-protein interaction provides a valuable framework for a better understanding of the functions of the histone lysine demethylases. All of these will be beneficial to a better understanding of demethylases and suggest how future research can be improved.
25 citations
TL;DR: 1H, 15N and 13C resonances of the Bright/ARID DNA-binding domain from the human JARID1C protein, a newly discovered histone demethylase belonging to the JmjC domain-containing protein family is assigned.
Abstract: We have assigned 1H, 15N and 13C resonances of the Bright/ARID DNA-binding domain from the human JARID1C protein, a newly discovered histone demethylase belonging to the JmjC domain-containing protein family.
13 citations
Patent•
11 Mar 2008
TL;DR: This article showed that histone H3K27 methylation is critical for repression of gene expression of HOX and other genes and that misregulation is associated with diseases including cancer including cancer.
Abstract: UTX and JMJD3 act as histone H3 demethylases, acting specifically on H3K27. Histone H3K27 methylation is critical for repression of gene expression of HOX and other genes. Misregulation of Η3K27 methylation is associated with diseases including cancer.
8 citations