Showing papers on "Murraya published in 2019"

Unsuitability of indigenous South American Rutaceae as potential hosts of Diaphorina citri

Abstract: Background 'Candidatus Liberibacter asiaticus' is transmitted by Diaphorina citri, an insect with a wide range of hosts in Rutaceae. Species related to Citrus occur in Brazilian forests where they may serve as hosts for psyllids and infested citrus orchards. Results The suitability of plants as hosts of D. citri was classified into four groups. Group I (high suitability): Citrus × aurantium 'Valencia', 'Citrus limonia', Murraya paniculata (syn. Murraya exotica L.) (Aurantioideae: Aurantieae) and Bergera koenigii (Aurantioideae: Clauseneae). Group II (intermediate to low suitability): Citrus (Poncirus) trifoliata 'Pomeroy', Citrus wintersii, Swinglea glutinosa (Aurantieae) and Clausena lansium (Clauseneae). Group III (not suitable): Aegle marmelos, Atalantia buxifolia, Citrus ('Microcitrus') sp. (Aurantieae) and Helietta apiculata (Amyridoideae). Group IV (non-hosts): Glycosmis pentaphylla (Clauseneae), Balfourodendron riedelianum, Casimiroa edulis, Esenbeckia febrifuga, Esenbeckia leiocarpa, Metrodorea stipularis, Zanthoxylum rhoifolium (Amyridoideae) and Dictyoloma vandellianum (Cneoroideae). Insects survived longer on newly differentiated leaves compared with fully expanded soft leaves. Psyllids either did not develop or did not survive for long on most Group IV species, all of which, with the exception of G. pentaphylla, occur naturally in Brazilian forests. Conclusion Citrus relatives occurring in forests near citrus orchards are not suitable hosts of D. citri and, therefore, do not contribute to huanglongbing spread. © 2018 Society of Chemical Industry.

Antihyperglycemic Activity of Murraya koenigii Leaves Extract on Blood Sugar Level in Streptozotocin-Nicotinamide Induced Diabetes in Rats

Abstract: The effects of Murraya koenigii leaves are very less studied in streptozotocin-nicotinamide (STZ-NA) induced diabetes rat model, in spite of several studies reported its antidiabetic effects in alloxan and STZ induced diabetes. The present study was undertaken to assess the effects of Murraya koenigii leaves extract on the blood sugar level (BSL) of STZ-NA diabetic rats. Experimental diabetes was induced by STZ injection intraperitoneally (i. p) after 30 min of NA injection i. p in all groups apart from normal control group. Group I (normal control) and Group II (diabetic control) rats received distilled water. Group III rats treated Metformin, Group IV and Group V rats treated Murraya koenigii aqueous extract and Murraya koenigii methanolic extract respectively. BSL and body weights of rats were measured at each week of the period of 28 days. Our results indicate that oral administration of Murraya koenigii reduces BSL significantly compared with the diabetic group. No weight loss was observed in all groups. The findings of the present study suggest that Murraya koenigii is proven as anti-diabetic agent in diabetic rats.

Molecular differentiation of the Murraya paniculata Complex (Rutaceae: Aurantioideae: Aurantieae).

Abstract: Orange jasmine has a complex nomenclatural history and is now known as Murraya paniculata (L.) Jack. Our interest in this common ornamental stemmed from the need to resolve its identity and the identities of closely related taxa as hosts of the pathogen ‘Candidatus Liberibacter asiaticus’ and its vector Diaphorina citri. Understanding these microbe-vector-plant relationships has been hampered by taxonomic confusion surrounding Murraya at both the generic and specific levels. To resolve the taxonomic uncertainty, six regions of the maternally-inherited chloroplastal genome and part of the nuclear-encoded ITS region were amplified from 85 accessions of Murraya and Merrillia using the polymerase chain reaction (PCR). Clustering used maximum parsimony (MP), maximum likelihood (ML) and Bayesian inference (BI). Chronograms were produced for molecular dating, and to test the monophyly of Murraya rigorously, using selected accessions of Murraya and 26 accessions of the Rutaceae and Simarubaceae. Sequence data from the ITS and chloroplastal regions suggest that Murraya paniculata (sensu (Swingle WT and Reece CR, The Citrus Industry, p. 190–430, 1967)) can be separated into four distinct but morphologically somewhat cryptic taxa: Murraya paniculata (sensu (Mabberley DJ, Taxon 65:366–371, 2016)), M. elongata, M. sumatrana and M. lucida. In addition, Murraya omphalocarpa was identified as a putative hybrid of M. paniculata and M. lucida with two geographically isolated nothovarieties representing reciprocal crosses. Murraya is monophyletic, and molecular dating suggests that it diverged from Merrillia during the Miocene (23–5 Ma) with this Murraya group speciating and dispersing during the Middle Miocene onwards. The accessions from Asia and Australasia used in this study grouped into biogeographical regions that match herbarium specimen records for the taxa that suggest natural allopatric distributions with limited overlap and hybridity. Murraya paniculata has been distributed around the world as an ornamental plant. The division of the Murraya paniculata complex into four species with a rare hybrid also confirms morphological studies.

Phytochemical Screening and Antioxidant Property of Aqueous Murraya Koenigii (Curry Leaf) Extract

Abstract: Murraya koenigii is commonly called as Curry Leaf, which belongs to the family Rutaceae. The leaves of Murraya koenigii was used for flavouring and spicing of food. These leaves were highly valued for their medicinal values and for characteristic aroma. There are many chemical compounds which helps the human beings in various ways. The compounds are non-nutritive and help in protection against diseases. Commonly curry leaves were accepted for various treatments in curing several disorders. Thus present study is an investigation to analyze the phytoscreening compounds present in the aqueous curry leaf and also further extended to find out its antioxidant activity using standard methods. The results of the aqueous curry leaf extract revealed the presence of phytochemical compounds such as Tannins, Saponins, Alkaloids, Proteins, Cardio glycosides and Phenols. Significant inhibitory antioxidant activity of DPPH assay was determined. Thus the IC50 value of DPPH assay was found to be 57.62±1.69% at 400μg/ml.

Efficacy of aqueous and cow-urine based biopesticides against Phytophthora nicotianae var. parasitica causing buckeye rot of tomato

Abstract: Buckeye rot of tomato, caused by Phytophthora nicotianae var. parasitica, is a serious threat to the crop production and has taken a heavy toll of the crop in India which affects mostly the fruits during both spring and winter season crops. In the present investigation, aqueous and cow-urine based leaf extracts of five plants viz., Lantana camara, Artemisia vulgaris, Murraya koenigii, Eucalyptus tereticornis and Melia azaderach were evaluated for their efficacy against the test pathogen under in vitro conditions. Out of five aqueous biopesticides, Eucalyptus tereticornis was found most effective with 51.48 per cent inhibition in mycelial growth of the pathogen followed by Artemisia vulgaris (48.52%), Lantana camara (44.81%) and Murraya koenigii (42.96%). Aqueous extract of Melia azedarach was least effective with 39.62 per cent mycelial growth inhibition. Among five cow-urine based biopesticides, Eucalyptus tereticornis was found most effective with 71.89 per cent inhibition in mycelial growth of the pathogen followed by Artemisia vulgaris (68.74%), Lantana camara (63.58%) and Murraya koenigii (59.51%). Cow-urine based botanical extract of Melia azedarach was least effective with 55.41 per cent mycelial growth inhibition.

Evaluation of antipyretic activity of alcoholic extract of Murraya koenigii leaves in rabbits

Abstract: Background: The present study was carried out to evaluate the antipyretic activity of alcoholic extract of Murraya koenigii leaves in rabbits by using PGE1 induced hyperpyrexia method. We found that not much work has been done on the antipyretic effect of this plant.Methods: Laboratory breed New Zealand strains of rabbits of either sex weighing 1000-1500 gm were used in the study. The animals were divided into five groups (n=6). The rabbits of all the groups were made febrile by injecting misoprostol (PGE1) subcutaneously in the dose of 100 mcg/kg. For group 1 and 2, normal saline 2ml/kg as control and aspirin 28mg/kg as standard were given respectively. Alcoholic extract of Murraya koenigii leaves in the doses of 200, 400 and 800mg/kg were administered in remaining three groups respectively. Rectal temperatures were recorded with help of digital thermometer for every 30 min after drug treatment.Results: Alcoholic extract of Murraya koenigii produced highly significant (p<0.001) antipyretic effect in 400 and 800 mg/kg doses. But onset of action was fast with 800mg/kg dose.Conclusions: This study concludes that Alcoholic extract of Murraya koenigii has fast onset of action and also exhibited sustained anti pyretic action in New Zealand rabbits.

Extraction of Nerium indicum Mill, Artocarpus heterophyllus Lam, Murraya koenigii Linn, Punica granatum Linn: Qualitative and quantitative assessment

Abstract: The objective of present work was to evaluate the qualitative and quantitative assessment of Nerium Indicum Mill, Artocarpus Heterophyllus Lam, Murraya Koenigii Linn and Punica Granatum Linn extract for the natural product present in them. These plants were having the rich source of alkaloid, glycoside, tannins, carbohydrates, saponin, flavonoids, proteins and amino acids and were used as anti-diabetic, antiulcer, anti-inflammatory, antitumor, anti-malarial and having wound healing potential. Extract of all four plants has been separated by the process of Soxhlet extraction by using 70% ethanol. The extract of Nerium Indicum mill, Artocarpus Heterophyllus Lam, Murraya Koenigii Linn, and Punica Granatum Linn was evaluated for qualitative and quantitative measurement of alkaloid, glycoside, tannins, carbohydrates, saponins, flavonoids, proteins and amino acids content. Different solvents was used includes petroleum ether, ethyl acetate, ethyl alcohol, distilled water and 70% ethanol for the extraction all four plants. Preliminary phyto-chemical screening was performed for extracts of Nerium Indicum Mill (NIL), Artocarpus Heterophyllus Lam (AHP), Murraya Koenigii Linn (MKL) and Punica Granatum Linn. (PGB). Identification test on extracts was shown the presence of alkaloid, glycoside, tannins, carbohydrates, saponin, flavonoids, proteins, volatile oil, steroids and amino acids content. All results denote that extracts of these four Indian plants having a rich source of phyto-constituents of interest.

Comparative effect of Beta blocker-Atenolol and Murraya koenigii (L.) spreng (Curry leaves) on cardiac enzyme (CK-MB) level in male albino rats.

Abstract: Murraya koenigii (L.) spreng (curry leaves) have traditionally been used for its various medicinal properties. The current study was conducted to assess the comparative effect of Murraya koenigii (L.) spreng (curry leaves) and market available beta blocker drug Atenolol on cardiac enzyme (CK-MB) level in male albino rats. Out of total 26 locally bred male Albino Wistar rats (180 to 200gm weight) two rats were treated with only voltral for dose adjustment. Remaining 24 rats were randomly categorized into following 1 control (C) group and 3 experimental groups Model (M), Test 1 (T1) and Test 2 (T2) containing 6 rats in each group. Rats in C group were orally fed by 0.9% saline solution while rats of M and both test groups T1 and T2 were orally treated with voltral tablet (30mg /kg body weight) for three weeks to increase the level of CK-MB heart enzyme. After voltral treatment rats in test group T1 were treated orally with Atenolol (30 mg/kg body weight) and T2 with Murraya koenigii (L.) spreng (curry leaves) extract (180 mg/kg body weight) for last three weeks. Results show that rats treated with Atenolol showed a decrease in level of heart enzyme as compare to M group, while Murraya koenigii (L.) spreng treated rats group T2 showed more significant decrease of heart enzyme (CK-MB) level as compared to M and T1 groups with significantly improved behavioral activity including increased locomotor activity, short-term memory and reduction in depression. These results demonstrate that natural herbal treatment by curry leaves extract play an effective role in lowering the cardiac enzyme (CK-MB) level to its normal range which helps reducing the risk of CVD and CHD.

Phytochemical Screening and Anti-inflammatory activity of Murraya Koenigii & Ficus Lacor roots in Carrageenan, Histamine and Serotonin induced paw edema in albino Wistar rats

Abstract: Objective: To perform the Phytochemical Screening & Anti-inflammatory activity of Murraya Koenigii & Ficus Lacor roots in Albino Wistar rats. Methods: The different Pharmacognostical parameters were evaluated as per standard procedure. The crude drug was evaluated for organoleptic properties shape, size, color, odor, taste. Preliminary Phytochemical Screening was carried out & finally Anti-inflammatory activity was evaluated by adopting different methods. Results: The extracts showed a marked Anti-inflammatory effect. The FLET fraction from Ficus lacor aerial roots showed maximum inhibition (75%) of Carrageenan induced edema (Table 3.12B), followed by FLPE and MKCF from Murraya koenigii roots (74 % and 70% approx) (p <0.001 for all). The inhibition of inflammation was comparable for all the fractions for any change in extent & percentage of inhibition at 30 min, 1hr, 2hr and 3hr. The anti-inflammatory effect induced via indomethacin gradually increased and reached at higher level (80.8%) after 3 hrs. It was maintained up to six hours. For, MKPE the inhibition was observed to be maximum at the end of 2 hrs and then tapered. MKEA, FLEA and FLCF showed minimum response that was constant throughout and insignificant. MKPE was slightly significant (55.1 %, p<0.05) which was more effective. Conclusion: The results of the study indicate that the extract of Murraya koenigii roots and Ficus lacor possesses strong Anti-inflammatory activity. This study also describes therapeutic effect of Murraya koenigii roots and Ficus lacor aerial roots in inflammation and arthritis which will give a new direction for the future scientific research.

Development and characterization of solid lipid nanoparticles containing Murraya koenigii leaves extract for management of diabetes mellitus

Abstract: Diabetes mellitus is a heterogeneous metabolic disorder characterized by common feature of chronic hyperglycemia with disturbance of carbohydrate, fat and protein metabolism. The main pathophysiology of diabetes mellitus is decrease in number of the beta cells of pancreas. The reduction in number of beta cells corresponds to the lack of insulin which results in a peripheral underuse and hepatic overproduction of glucose which results hyperglycemia (Deepthi et al., 2017). Two major types of diabetes mellitus are: Type-I Insulin dependent diabetes mellitus (IDDM), characterized by the loss of insulin-producing beta cells of the islets of langerhans in the pancreas leading to a deficiency of insulin. Type-II Non insulin dependent diabetes mellitus (NIDDM), there is no loss or moderate reduction in beta cell mass; insulin in circulation is low (Olokoba et al., 2012). Conventional drug delivery systems utilized in the treatment of diabetes extend a number of drawbacks such as transient leucopenia, agranulocytosis, allergy, edema, weight gain, lipodystrophy occurs at injection site etc (Piero et al., 2014).

Physiological Response of Capsicum annuum L. to Aqueous Extracts of Allelopathic Plants: A Case of Tithonia rotundifolia and Murraya koenigii

Abstract: The present study evaluated the allelopathic potential of fresh shoot aqueous extracts of Tithonia rotundifolia (FSET) and Murraya koenigii (FSEM) on the germination of seeds, growth, chlorophyll, ascorbic acid and percentage crude protein accumulation of Capsicum annuum. The laboratory experimental results showed that seed germination and juvenile seedling growth of C. annuum were significantly retarded by both FSEM and FSET. The radicle growth was more susceptible to the phytotoxicity of both extracts than the plumule growth. Both plants extract had a concentration-dependent inhibitory effect on the seedling growth of the recipient crop while the FSET was more phytotoxic than FSEM. However, this retardatory effect of aqueous extracts on growth observed in the laboratory was reversed in the soil-cultured experiments such that application of FSEM significantly enhanced all the studied growth parameters (shoot height, number of leaves, leaf area, leaf area ratio, shoot fresh and dry weight, root fresh and dry weight, chlorophyll a, chlorophyll b, total chlorophyll, ascorbic acid content in the shoot and percentage crude protein in the shoot and fruits). Likewise, FSET application significantly increased the leaf area, total chlorophyll and percentage crude protein accumulation in the shoot and fruits of the recipient crop at P<.05. This study then emphasizes the fact that T. rotundifolia and M. koenigii are allelopathic plants. The increased ascorbic acid and percentage crude protein accumulation in the aqueous extract-treated crop could, apart from enhancing the nutritional benefits of the test crop, be an adaptive mechanism evolved by the crop to overcome the allelopathic stress posed by the application of the aqueous extracts. These findings therefore suggest that allelochemicals in the aqueous extract of M. koenigii and T. rotundifolia could serve as biofertilizers for boosting the production of C. annuum.

Evaluation of antimicrobial activity of ethanolic extract of Murraya koenigii against S. mutans

Abstract: Periodontal disease is a bacterial infection that affects the gums and bone supporting the teeth. The present study was aimed to evaluate the Antimicrobial activity of Murraya koenigii extract against Streptococcus mutans. Ethanol extract was subjected to phytochemical screening, thin layer chromatography, total phenolic content, antioxidant activity, antimicrobial activity. Phytochemical analysis of the extract of Murraya koenigii indicated the presence of phytochemicals such as flavvanoids, phenolics, alkaloids, and terpenoids. Thin layer chromatography was performed. Antioxidant assays were evaluated by assessing DPPH radical scavenging activity against standard butylated hydroxyanisole (BHA). From this study it can be concluded that Murraya koenigii shows significant antibacterial effect against gram positive S mutans. The Murraya koenigii were found to contain gallic acid and other phenolics which may be responsible for antibacterial activity. The studies will be explored for development of novel formulation using Murraya koenigii extract for periodontal diseases.

Studi Literature Essential oil bioactivity of Curry leavess (Murraya koeginii ) as slimming aromatherapy

Abstract: Curry leaves is an aromatic plant that contained essential oil. The essential oil mostly has potential as aromatherapy. The aim of this research is to separate the compounds in the essential oil of Kari leaf through fractionation technique and to analyze the potential of the active compound as slimming aromatherapy. The sliming aromatherapy of the obtained each separated essential is evaluated in vivo in male Sprague-Dawley (SD) rats by aromatherapy and inhalation technique. Twenty four rats were used and divided into four groups, namely positive control, negative control, inhale the crude essential oil and inhale the active compound of essential oil. This article contains the research to be done.

Phytochemical Screening of Murraya koenigii (L.) Spreng

Abstract: Murraya koenigii (L.) Spreng., commonly known as curry leaf tree is well known in Ayurvedic medicinal system for its varied pharmacological activities like anticancer activity, antioxidant activity, anti-inflammatory activity, anthelmintic activity, antidiabetic and antimicrobial activity. The leaves of this tree had been commonly used to enhance the flavour in culinary purposes for its specific taste and aroma. It’s Stem part is commonly used as datun for oral health care purposes.The secondary metabolites present in the plant are also known for its different pharmacological activities. The present study focuses on preliminary qualitative phytochemical Screening and analysis from the hydroalcoholic extracts of root, stem and leaves part which detected the presence of alkaloids, phenols, flavonoids, terpenoids, steroids and tannins in the prepared hydroalcoholic extracts using Soxhlet extraction method.

Effects of Aqueous Leaf Extracts of Murraya koenigii on Learning and Memory in Mice

Abstract: Curry (Muraya koenigii) leaf is an essential leafy spice used widely in cuisine for its distinct flavor and for other medicinal purposes: Analgesic, antidysenteric, antioxidant and in regulating fertility. The Morris water maze was used to study the effects of aqueous extracts of M. koenigii (curry) leaf on learning and memory. Aqueous leaf extracts of M. koenigii (80mg/kg, p.o.) was administered to 7 CD1 strain of mice (18-28 g body weight) while the control group received 0.1 ml/10 kg body weight of distilled water (orally) for 10 days before behavior was assessed. All mice were tested in the Morris water maze for 8 days: At 4 trials per day and 60 seconds per trial. Day 1-3 were for acquisition training, day 4-6 reversal training, day 7, the probe trail and day 8 visible platform task. Results indicate that swim latency were not significantly different between the groups during acquisition and reversal training. The retention quadrant duration was significantly higher for the M. koenigi-treated mice compare to the control (P<0.05). The mice treated with M. koenigi showed a negative weight gain, indicating weight loss (p< 0.05). Therefore the aqueous extracts of M. koenigi improved visuospatial memory in the mice and decreased body weight.

Evaluation of antidiabetic efficacy of Murraya koenigii on Streptozotocin induced diabetes in experimental rats

Abstract: Background: The medicinal plant Murraya koenigii shown to have a wide variety of pharmacological activities (hypoglycemic and hypolipidemic). Objective of this study is the present study was designed to evaluate Antidiabetic and Hypolipidemic property of Murraya koenigii in experimentally induced diabetes in rats.Methods: Experimental diabetes was produced with single dose of Streptozotocin (STZ): 45 mg/kg IP. The rats were randomly allocated in various groups for 37 days. After the confirmation of diabetes on 7th day (>200 mg/dl), hydroalcoholic extract of Murraya koenigii (500 mg/kg) was administered orally to experimental rats from day 7th day and continued for 37 days thereafter. Various antidiabetic (Glucose, HbA1C), metabolic (Lipid profile), safety (pancreatic lipase, Creatinine, SGPT, Histopathology of Liver and Kidney) were evaluated in various group.Results: Efficacy of Murraya koenigii was observed on various parameter of diabetes. Administration of STZ resulted in a significant decrease in diabetic changes (increase in blood glucose, HbA1C), altered lipid profile (p<0.01) in the Control group rats as compared to sham group. Murraya koenigii treatment demonstrated significant antidiabetic indicated by restoration of blood glucose, HbA1C level (p<0.01) compared to Control group. In addition, Murraya koenigii also documented hypolipidemic property of test drug. As per biochemical assessment of Pancreatic lipase, Serum creatinine, SGPT and Histopathological report, the test drug reduce the pancreatic, liver and renal marker and also showed safe to pancreas, Liver and kidney. The histopathological assessment of the liver and kidney confirmed the biochemical findings.Conclusions: The study concluded that the Murraya koenigii possess antidiabetic efficacy.

Pharmacognostical evaluation of Murraya roots

Abstract: Pharmacognostical evaluation is the first and foremost step to determine identity and assess the quality and purity of the crude drug. Murraya koenigii Spreng (Rutaceae), commonly known as Curry leaf plant or Mitha Neem in Hindi is a highly valued plant for its medicinal value and characteristic aroma. Leaves of the plants are extensively used as spice and condiment in India and other tropical countries. Present research work includes study of macroscopic, microscopic study of the root include anatomy of the thin root, anatomy of the thick root and microscopy of the powdered root. Physiochemical studies were done by using WHO recommended parameters. Photographs at different magnifications were taken with Nikon Labphot-2 microscopic unit. These findings will be useful towards establishing pharmacognostical standards and preparation of monograph of the root of Murraya koenigii.

Growth and Yield Responses of Curry Plant (Murraya koenigii) to Plant Row Spacing in an Utisol

Abstract: Curry plant (Murraya koenigii) is one of the most important vegetable spices crops use for food, spice, medicinal and industrial purposes in Nigeria. However, curry plant production in commercial scale in Nigeria is highly constrained by several factors: it is not cultivated in regular farming system mainly due to lack of planting materials and improper plant spacing of this crop among others. Therefore, field experiments were conducted to determine the effect of different levels of plant row spacing on growth and yield of Curry plant (Murraya koenigii) in Cross River University of Technology, Teaching and Research farm, Department of Agronomy, Obubra, Cross River state, Nigeria in 2017 and 2018 cropping seasons. The experimental design was a randomized complete block design. Treatments were nine plant row spacing (70 x 50 cm, 70 x 70 cm, 80 x 40 cm, 80x60 cm, 90 x 30 cm, 100 x 50 cm, 100 x 70 cm, 100 x100 cm and 100 x130 cm) inter and intra row respectively with four replications. Results showed that plant row spacing have significantly affect the growth and yield of Curry plant. Closer plant row spacing of 70 x 50 cm significantly promoted the tallest plant height and highest leaf area inde x values of curry plant. Fresh and dry leaf yield on per hectare basis were significantly higher in closer plant row spacing than wider row spacing. The highest fresh and dry leaves yield per hectare at 20 weeks after planting (WAP) of 0.9742t/ha and 0.9871t/ha were obtained in closer row spacing of 70 x 50 cm in 2017 and 2018 cropping seasons respectively . The number of leaves and branches per plant and their fresh leaf yield and dry matter of plant fractions were higher in wider plant row spacing plots. The wide plant row spacing of 100 x 130 cm produced the highest fresh leaf yield per plant of 88.54 g in 2017 and 83.46 g in 2018 cropping seasons. Based on this result farmers are advised to cultivate Curry plant (Murraya koenigii) using the plant row spacing of 70 x 50 or 80 x 60 cm for optimum growth and yield per hectare of the crop under the utisoil condition.

Phytochemical Analysis of Wild Meethi Neem [Murraya koenigii (L.) Spreng] Collections of Himachal Pradesh

Abstract: Mountains support an estimated one-third of terrestrial species diversity (Körner, 2004) and host half of all 34 global biodiversity hotspots (Chape et al., 2008). The Himalayan mountains is well known as a source of medicinal and aromatic plants, which contain variety of bioactive agents. Since the beginning of human civilization, medicinal plants have been used by mankind for its therapeutic value. Medicinal plants are generally tree, shrub, herb, annuals, biennial, tubers, rhizomes and climbers containing bioactive compounds. Some of the medicinal plant includes tulsi, neem, babul, lavender, brahmi, curry patta (Meethi Neem), datura, satavar, arjun and sarpagandhha. Meethi Neem (Murrayakoenigii) is one among them which has been extensively used for culinary International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 8 Number 12 (2019) Journal homepage: http://www.ijcmas.com

Pharmacognostical, Preliminary phytochemical screening and Antibacterial activity of methanol Extract of Murraya koenigii

Abstract: The methanol extract of murraya koenigii (leaf) were investigated for their antibacterial activity against staphylococcus aureus using cup plate agar diffusion method and methanol extract produces best results. Various investigations like physiochemical standard, preliminary phytochemical screening of the plant was carried out and parameters were reported. The present paper deals with investigation of various phytochemical present in methanol extract and in vitro antibacterial activity against the s.aureus. The literature survey does not exist antibacterial activity of murraya koenigii (leaf) in methanol extract against s.aureus. It is concluded that the plant studied may source of antibacterial plant.