Showing papers by "Andrew J. Watson published in 2005"

Ocean acidification due to increasing atmospheric carbon dioxide

Abstract: The oceans cover over two-thirds of the Earth’s surface. They play a vital role in global biogeochemical cycles, contribute enormously to the planet’s biodiversity and provide a livelihood for millions of people. (...)

Ecosystem dynamics based on plankton functional types for global ocean biogeochemistry models

Abstract: Ecosystem processes are important determinants of the biogeochemistry of the ocean, and they can be profoundly affected by changes in climate. Ocean models currently express ecosystem processes through empirically derived parameterizations that tightly link key geochemical tracers to ocean physics. The explicit inclusion of ecosystem processes in models will permit ecological changes to be taken into account, and will allow us to address several important questions, including the causes of observed glacial-interglacial changes in atmospheric trace gases and aerosols, and how the oceanic uptake of CO2 is likely to change in the future. There is an urgent need to assess our mechanistic understanding of the environmental factors that exert control over marine ecosystems, and to represent their natural complexity based on theoretical understanding. We present a prototype design for a Dynamic Green Ocean Model (DGOM) based on the identification of (a) key plankton functional types that need to be simulated explicitly to capture important biogeochemical processes in the ocean; (b) key processes controlling the growth and mortality of these functional types and hence their interactions; and (c) sources of information necessary to parameterize each of these processes within a modeling framework. We also develop a strategy for model evaluation, based on simulation of both past and present mean state and variability, and identify potential sources of validation data for each. Finally, we present a DGOM-based strategy for addressing key questions in ocean biogeochemistry. This paper thus presents ongoing work in ocean biogeochemical modeling, which, it is hoped will motivate international collaborations to improve our understanding of the role of the ocean in the climate system.

Bio‐optical feedbacks among phytoplankton, upper ocean physics and sea‐ice in a global model

Abstract: [1] Phytoplankton biomass modifies the penetration of light and impacts the physical properties of the upper ocean. We quantify these impacts and the feedbacks on phytoplankton biomass for the global ocean using an Ocean General Circulation Model coupled to an ocean biogeochemistry model. Phytoplankton biomass amplifies the seasonal cycle of temperature, mixed layer depth and ice cover by roughly 10%. At mid and high latitudes, surface temperature warms by 0.1–1.5°C in spring/summer and cools by 0.1–0.3°C in fall/winter. In the tropics, phytoplankton biomass indirectly cools the ocean surface by 0.3°C due to enhanced upwelling. The mixed layer stratifies by 4–30 m everywhere except at high latitudes. At high latitudes, the sea-ice cover is reduced by up to 6% in summer and increased by 2% in winter, leading to further feedbacks on vertical mixing and heat fluxes. Physical changes drive a positive feedback increasing phytoplankton biomass by 4–12% and further amplifies the initial physical perturbations.

A comparison of multiple regression and neural network techniques for mapping in situ pCO2 data

Abstract: Using about 138 000 measurements of surface p CO 2 in the Atlantic subpolar gyre (50–70°N, 60–10°W) during 1995–1997, we compare two methods of interpolation in space and time: a monthly distribution of surface p CO 2 constructed using multiple linear regressions on position and temperature, and a self-organizing neural network approach. Both methods confirm characteristics of the region found in previous work, i.e. the subpolar gyre is a sink for atmospheric CO 2 throughout the year, and exhibits a strong seasonal variability with the highest undersaturations occurring in spring and summer due to biological activity. As an annual average the surface p CO 2 is higher than estimates based on available syntheses of surface p CO 2 . This supports earlier suggestions that the sink of CO 2 in the Atlantic subpolar gyre has decreased over the last decade instead of increasing as previously assumed. The neural network is able to capture a more complex distribution than can be well represented by linear regressions, but both techniques agree relatively well on the average values of p CO 2 and derived fluxes. However, when both techniques are used with a subset of the data, the neural network predicts the remaining data to a much better accuracy than the regressions, with a residual standard deviation ranging from 3 to 11 μatm . The subpolar gyre is a net sink of CO 2 of 0.13 Gt-C yr −1 using the multiple linear regressions and 0.15 Gt-C yr −1 using the neural network, on average between 1995 and 1997. Both calculations were made with the NCEP monthly wind speeds converted to 10 m height and averaged between 1995 and 1997, and using the gas exchange coefficient of Wanninkhof. DOI: 10.1111/j.1600-0889.2005.00164.x

Potential and limitations of bovine-specific arrays for the analysis of mRNA levels in early development: preliminary analysis using a bovine embryonic array.

Abstract: New insights into the early development of large mammals are becoming available through the measurement of differential mRNA levels in oocytes and preimplantation embryos. These advances in knowledge are rapidly picking up in pace, mainly owing to the advantages brought by new molecular biology approaches being developed. The possibility of amplifying the starting material and therefore making measurements in single embryo units is now feasible. With these tools, the evaluation of variations in gene expression patterns during the preimplantation period or the impact of culture on mRNA levels is now possible. However, it is important to keep in mind that these methods still have limitations associated with sample preparation or the use of the appropriate controls. Even proper methods of analysis are very important to achieve the full benefit of the application of these tools. The present paper describes some of the potential, as well as limitations, of mRNA level analysis in early embryos, especially for microarray analysis. We have generated a bovine cDNA array (>2000 clones) that contains expressed sequence tags (ESTs) collected from various preimplantation development stages. Using this chip, we have initiated the characterisation of global mRNA level patterns of several key developmental stages from the immature oocyte to the blastocyst stage. As expected, the hybridisation results indicate very different expression profiles involving hundreds of genes when comparing oocyte and blastocyst samples to a reference mRNA sample made from a pool of ESTs from pooled somatic tissues. Although this array is still in its preliminary stage and the EST bank has not been processed to contain only unigenes, it is already a very useful tool for discovering candidate genes that may play important roles during early embryonic life.

Iron and mixing affect biological carbon uptake in SOIREE and EisenEx, two Southern Ocean iron fertilisation experiments

Abstract: This study explores the changes in the surface water fugacity of carbon dioxide ( f CO 2 ) and biological carbon uptake in two Southern Ocean iron fertilisation experiments with different hydrographic regimes. The Southern Ocean Iron Release Experiment (SOIREE) experiment was carried out south of the Antarctic Polar Front (APF) at 61°S, 141°E in February 1999 in a stable hydrographic setting. The EisenEx experiment was conducted in a cyclonic eddy north of the APF at 48°S, 21°E in November 2000 and was characterised by a rapid succession of low to storm-force wind speeds and dynamic hydrographic conditions. The iron additions promoted algal blooms in both studies. They alleviated algal iron limitation during the 13-day SOIREE experiment and probably during the first 12 days of EisenEx. The f CO 2 in surface water decreased at a constant rate of 3.8 μatm day −1 from 4 to 5 days onwards in SOIREE. The f CO 2 reduction was 35 μatm after 13 days. The evolution of surface water f CO 2 in the iron-enriched waters (or ‘patch’) displayed a saw tooth pattern in EisenEx, in response to algal carbon uptake in calm conditions and deep mixing and horizontal dispersion during storms. The maximum f CO 2 reduction was 18–20 μatm after 12 and 21 days with lower values in between. The iron-enriched waters in EisenEx absorbed four times more atmospheric CO 2 than in SOIREE between 5 and 12 days, as a result of stronger winds. The total biological uptake of inorganic carbon across the patch was 1389 ton C (±10%) in SOIREE and 1433 ton C (±27%) in EisenEx after 12 days (1 ton=10 6 g). This similarity probably reflects the comparable size of the iron additions, as well as algal growth at a similar near-maximum growth rate in these regions. The findings imply that the different mixing regimes had less effect on the overall biological carbon uptake across the iron-enriched waters than suggested by the evolution of f CO 2 in surface water.

Mitogen-activated protein kinase (MAPK) blockade of bovine preimplantation embryogenesis requires inhibition of both p38 and extracellular signal-regulated kinase (ERK) pathways.

Abstract: Blastocyst formation, as a critical period during development, is an effective indicator of embryonic health and reproductive efficiency. Out of a number of mechanisms underlying blastocyst formation, highly conserved mitogen-activated protein kinase (MAPK) signaling has emerged as a major mechanism involved in regulating murine preimplantation embryo development. The objective of our study was to ascertain the role of MAPK signaling in regulating bovine development to the blastocyst stage. Using reverse transcriptase PCR and immunohistochemical staining procedures we have demonstrated that mRNA transcripts and polypeptides encoding p38 MAPK pathway constituents are detectable in preimplantation bovine embryos from the one-cell to the blastocyst stage. Further, the effects on bovine embryo development following inhibition of p38 [alpha]/{szligbeta} and extracellular signal-regulated kinase (ERK) signaling by treatment with SB220025 and U0126, respectively, were investigated. Eight-cell bovine embryos (50 per group; three replicates) were placed into treatments consisting of synthetic oviductal fluid (SOF) medium: SOF + SB202474 (inactive analogue), SOF + SB220025, SOF + U0124 (inactive analogue), SOF + U0126, and SOF + SB220025 + U0126. Inhibition of p38 MAPK or ERK signaling individually did not affect development to the blastocyst stage. However, when both pathways were blocked simultaneously there was a significant reduction (P < 0.05) in blastocyst formation, cell number and immunofluorescence of phosphorylated downstream pathway constituents. We have determined that, in variance to what was observed during murine preimplantation development, bovine early embryos progress at normal frequencies to the blastocyst stage in the presence of p38 MAPK inhibitors.

Intermediate water from the Greenland Sea in the Faroe Bank Channel : spreading of released sulphur hexafluoride

Abstract: The Faroe Bank Channel is the deepest passage for dense water leaving the Nordic Seas into the North Atlantic. The contribution to this part of the Greenland-Scotland Overflow by intermediate water from the Greenland Sea is investigated by the tracer sulphur hexafluoride (SF6) that was released into the central Greenland Sea in summer 1996. Continuous monitoring has since traced it around the Nordic Seas and into the connecting areas. It was observed for the first time close to the Faroe Islands in early 1999, indicating a transport time from the Greenland Sea of around 2.5 years. This study estimates that approximately 16 kg of SF6 had passed the Faroe Bank Channel by the end of 2002, that is 5% of the total amount released. Both the arrival time and the amount of exported SF6 deduced from the observations are consistent with the results from a numerical ocean model simulating the tracer release and spreading.

Modeling past atmospheric CO2: Results of a challenge

Abstract: The models and concepts used to predict future climate are based on physical laws and information obtained from observations of the past. New paleoclimate records are crucial for a test of our current understanding. The Vostok ice core record [Petit et al., 1999] showed that over the past 420 kyr (1 kyr = 1000 years), Antarctic climate and concentrations of the greenhouse gases carbon dioxide (CO2) and methane (CH4) were tightly coupled. In particular, CO2 seemed to be confined between bounds of about 180 ppmv (parts per million by volume) in glacial periods and 280 ppmv in interglacials; both gases rose and fell with climate as the Earth passed through four glacial/interglacial cycles.

Can limited ocean mixing buffer rapid climate change

Abstract: It has been argued that diapycnal mixing has a strongly stabilizing role in the global thermohaline circulation (THC). Negative feedback between THC transport and low-latitude buoyancy distribution is present in theory based on thermocline scaling, but is absent from Stommel’s classical model. Here, it is demonstrated that these two models can be viewed as opposite limits of a single theory. Stommel’s model represents unlimited diapycnal mixing, whereas the thermocline scaling represents weak mixing. The latter limit is more applicable to the modern ocean, and previous studies suggest that it is associated with a more stable THC. A new box model, which can operate near either limit, is developed to enable explicit analysis of the transient behaviour. The model is perturbed from equilibrium with an increase in surface freshwater forcing, and initially behaves as if the only feedbacks are those present in Stommel’s model. The response is buffered by any upper ocean horizontal mixing, then by propagation of salinity anomalies, each of which are stabilizing mechanisms. However, negative feedback associated with limited diapycnal mixing only prevents thermohaline catastrophe in a modest parameter domain. This is because the time-scale associated with vertical advective-diffusive balance is much longer than the time required for the THC to change mode. The model is then tuned to allow equilibrium THC transport to be independent of the rate of mixing. The equilibrium surface salinity difference controls the classical THC-transport/salinity positive feedback, whereas the equilibrium interior density difference controls the mean-flow negative feedback. When mixing is strong, unrealistic vertical homogenization occurs, causing a convergence in surface and interior meridional gradients. This reduces positive feedback, and increases stability, in the tuned model. Therefore, Stommel’s model appears to overestimate, rather than underestimate, THC stability to high-frequency changes in forcing.

Southern Ocean blooms promote atmospheric CO2 uptake

Abstract: Large parts of the Southern Ocean have low primary productivity, often due to lack of sufficient iron. Seasonal algal blooms occur locally, eg. in the wake of islands, in ice-free waters near Antarctica and near frontal systems. Surface iron concentrations in these blooms are relatively high [De Baar et al., 1995; Buccarielli et al., 2001]. Here we will discuss how such algal blooms influence the CO2 air-sea gradient and CO2 air-sea exchange relative to waters outside the bloom. The presentation will include results from the Crozet Plateau, the Polar Front at 6°W, and the Weddell Sea.