Annelie Mollbrink

TL;DR: By positioning histological sections on arrayed reverse transcription primers with unique positional barcodes, this work demonstrates high-quality RNA-sequencing data with maintained two-dimensional positional information from the mouse brain and human breast cancer.

TL;DR: To define the cellular composition and architecture of cutaneous squamous cell carcinoma (cSCC), single-cell RNA sequencing with spatial transcriptomics and multiplexed ion beam imaging from a series of human cSCCs and matched normal skin were combined.

TL;DR: Spatiotemporal transcriptomics in ALS spinal cord models reveals dynamics of disease-driven gene regulation and identifies pathway dynamics, distinguish regional differences between microglia and astrocyte populations at early time points, and discern perturbations in several transcriptional pathways shared between murine models of ALS and human postmortem spinal cords.

TL;DR: A protocol describing how to apply the Spatial Transcriptomics technology to mammalian tissue is presented, which combines histological staining and spatially resolved RNA-sequencing data from intact tissue sections.

TL;DR: It is demonstrated that, in mice, longitudinal bone growth during fetal and neonatal periods relies on the gradual consumption of chondroprogenitors, whereas in adults, a stem cell niche is formed allowing renewing ofChondro Progenitors and leading to formation of large, stable monoclonal columns of chONDrocytes.