A
Arnold L. Demain
Researcher at Drew University
Publications - 425
Citations - 21576
Arnold L. Demain is an academic researcher from Drew University. The author has contributed to research in topics: Streptomyces clavuligerus & Clostridium thermocellum. The author has an hindex of 66, co-authored 424 publications receiving 20140 citations. Previous affiliations of Arnold L. Demain include Massachusetts Institute of Technology & Merck & Co..
Papers
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Tetanus toxin production in soy-based medium: nutritional studies and scale-up into small fermentors
TL;DR: This work aims to further improve the soy‐based medium, devoid of animal and dairy products, for a production of tetanus toxin by nutritional studies and to scale‐up the Clostridium tetani process into small fermentors.
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Unexpected enhancement of β-lactam antibiotic formation in Streptomyces clavuligerus by very high concentrations of exogenous lysine
TL;DR: It was surprised to note that as the concentration was further increased, the organism responded by producing even higher titers of antibiotics.
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Enzymatic Synthesis of a Sulfur-Analog of Penicillin Using the “cyclase” of Cephalosporium Acremonium
TL;DR: By the use of a semi-purified preparation of isopenicillin N synthetase (“cyclase”) from Cephalosporium acremonium, a new penicillin was prepared enzymatically, which will be economically used in the future to generate new β-lactam antibiotics, now made by expensive synthetic chemistry.
Journal ArticleDOI
Characterization and complementation of a cephalosporin-deficient mutant of Streptomyces clavuligerus NRRL 3585.
Jacqueline Piret,Bertha Resendiz,Bertha Resendiz,Bernd Mahro,Jinyou Zhang,Eugene Serpe,Jorge Romero,Neal Connors,Arnold L. Demain +8 more
TL;DR: A mutant of Streptomyces clavuligerus NRRL 3585 is characterized which is almost completely blocked in cephalosporin biosynthesis and exhibits depressed activities of both the delta(l-alpha-aminoadipyl)-l-cysteinyl-d-valine (ACV) synthetase and cyclase enzymes of the cep Halosphorin pathway.
Patent
Synthesis of isopenicillin derivatives in the absence of living cells
TL;DR: Isopenicillin derivatives having antimicrobial properties and a general formula of: ##STR1## where R, R1, and R2 are hydrogen, methyl, ethyl, propyl, isopropyl, or halogenated methyl, ethanol, or propyl radicals and R3 is ##STR2## are produced in a cell-free system using an extract from Cephalosporium acremonium.