[신간의 별자리x] 우리/미술, 그리고 ‘슬픔의 박물관’

J. Appl. Cryst.の発刊に際して

Yuming Yang,†,§ Qiang Zhao,‡,§ Wei Feng,† and Fuyou Li*,† †Department of Chemistry and State Key Laboratory of Molecular Engineering of Polymers and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, P. R. China ‡Key Laboratory for Organic Electronics and Information Displays (KLOEID) and Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, Nanjing 210046, P. R. China.

Luminescent chemodosimeters for bioimaging.

: The unpolarized absorption and circular dichroism spectra of the fundamental vibrational transitions of the chiral molecule, 4-methyl-2-oxetanone, are calculated ab initio. Harmonic force fields are obtained using Density Functional Theory (DFT), MP2, and SCF methodologies and a 5S4P2D/3S2P (TZ2P) basis set. DFT calculations use the Local Spin Density Approximation (LSDA), BLYP, and Becke3LYP (B3LYP) density functionals. Mid-IR spectra predicted using LSDA, BLYP, and B3LYP force fields are of significantly different quality, the B3LYP force field yielding spectra in clearly superior, and overall excellent, agreement with experiment. The MP2 force field yields spectra in slightly worse agreement with experiment than the B3LYP force field. The SCF force field yields spectra in poor agreement with experiment.The basis set dependence of B3LYP force fields is also explored: the 6-31G* and TZ2P basis sets give very similar results while the 3-21G basis set yields spectra in substantially worse agreements with experiment. jg

Ab initio calculation of vibrational absorption and circular dichroism spectra using density functional force fields

Recently, more than 1000 large intergenic noncoding RNAs (lincRNAs) have been reported. These RNAs are evolutionarily conserved in mammalian genomes and thus presumably function in diverse biological processes. Here, we report the identification of lincRNAs that are regulated by p53. One of these lincRNAs (lincRNA-p21) serves as a repressor in p53-dependent transcriptional responses. Inhibition of lincRNA-p21 affects the expression of hundreds of gene targets enriched for genes normally repressed by p53. The observed transcriptional repression by lincRNA-p21 is mediated through the physical association with hnRNP-K. This interaction is required for proper genomic localization of hnRNP-K at repressed genes and regulation of p53 mediates apoptosis. We propose a model whereby transcription factors activate lincRNAs that serve as key repressors by physically associating with repressive complexes and modulate their localization to sets of previously active genes.

A Large Intergenic Noncoding RNA Induced by p53 Mediates Global Gene Repression in the p53 Response

A ratiometric fluorescent probe containing a comarin-pyrazoline fluorophore and a 2,4-dinitrobenzenesulfonyl (DNBS) group for thiols has been developed. The probe can fast recognize thiols under both one-photon and two-photon excitations in PBS solution with low detection limits (one-photon 10−8 M and two-photon 10−7 M). The sensing mechanism was confirmed by using ESI-MS and fluorescent spectra. Moreover, the probe was successfully used for the fluorescent imaging of living cells and calf serum.

A ratiometric fluorescent probe with DNBS group for biothiols in aqueous solution

Synthesis of ferrocenyl pyrazole-containing chiral aminoethanol derivatives and their inhibition against A549 and H322 lung cancer cells

A novel lipid droplets-targeting ratiometric fluorescence probe for hypochlorous acid in living cells

A simple pyrazoline-based highly sensitive fluorescent probe for Zn2+ in aqueous solution and biological Zn2+ detection has been developed The probe displays a good selective fluorescence enhancement (13-fold) for Zn2+ over various metal ions The new probe is cell-permeable and can be successfully used to image intracellular Zn2+ in living neuron cells

A simple pyrazoline-based fluorescent probe for Zn2+ in aqueous solution and imaging in living neuron cells

An efficient “turn-on” rodamine-based fluorescent probe for intracellular Cu 2+ image in living HeLa cells has been developed. The fluorescent probe exhibits high Cu 2+ selectivity and excellent sensitivity (limit of detection is 1.2 × 10 −8  M) in CH 3 CN/HEPES (1/1, v/v) buffered solution and an immediate fluorescent response toward Cu 2+ under physiological conditions. Moreover, the fluorescent probe effectuates the naked-eye detection of Cu 2+ .

Bao-Xiang Zhao

Papers

A ratiometric fluorescent probe with DNBS group for biothiols in aqueous solution

Synthesis of ferrocenyl pyrazole-containing chiral aminoethanol derivatives and their inhibition against A549 and H322 lung cancer cells

A novel lipid droplets-targeting ratiometric fluorescence probe for hypochlorous acid in living cells

A simple pyrazoline-based fluorescent probe for Zn2+ in aqueous solution and imaging in living neuron cells

An effective “turn-on” rodamine-based fluorescent chemosensor for Cu (II) in living cells