Benjamin L. Parker

TL;DR: A new paradigm by which tissue crosstalk during exercise can exert systemic biological effects is identified, identified by employing arteriovenous balance studies across the contracting human limb to identify several novel candidate myokines released into circulation independently of classical secretion.

TL;DR: It is found that simulating the postnatal switch in metabolic substrates from carbohydrates to fatty acids promoted a switch in metabolism, DNA damage response, and cell cycle arrest in hPSC-CM, and under optimized maturation conditions, functional and molecular characterization revealed that a switch to fatty acid metabolism was a central driver of cardiac maturation.

TL;DR: The data demonstrate that peptide-centric approaches coupled to novel mass spectrometric fragmentation techniques may be suitable for application to eukaryotic glycoproteins for simultaneous elucidation of glycan structures and peptide sequence.

TL;DR: A global analysis of protein phosphorylation in human skeletal muscle biopsies from untrained healthy males before and after a single high-intensity exercise bout revealed 1,004 unique exercise-regulated phosphosites on 562 proteins, exposing the unexplored complexity of acute exercise signaling.

TL;DR: A protocol for the selective enrichment of SA-containing glycopeptides using a combination of titanium dioxide (TiO2) and hydrophilic interaction liquid chromatography (HILIC) and the efficiency of the method is illustrated by the identification of 1,632 unique formerly sialylated glycoproteins.