M
Martin R. Larsen
Researcher at University of Southern Denmark
Publications - 301
Citations - 15547
Martin R. Larsen is an academic researcher from University of Southern Denmark. The author has contributed to research in topics: Phosphorylation & Proteome. The author has an hindex of 62, co-authored 262 publications receiving 13799 citations. Previous affiliations of Martin R. Larsen include Children's Medical Research Institute & Technical University of Denmark.
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Journal ArticleDOI
Highly Selective Enrichment of Phosphorylated Peptides from Peptide Mixtures Using Titanium Dioxide Microcolumns
TL;DR: This work reports a highly selective enrichment procedure for phosphorylated peptides based on TiO2microcolumns and peptide loading in 2,5-dihydroxybenzoic acid (DHB), and demonstrates that this new procedure was more selective for binding phosphorylation peptides than IMAC using MALDI mass spectrometry.
Journal ArticleDOI
Highly selective enrichment of phosphorylated peptides using titanium dioxide
TL;DR: A protocol for selective phosphopeptide enrichment using titanium dioxide (TiO2) chromatography is described, well suited for the characterization of phosphoproteins from both in vitro and in vivo studies in combination with mass spectrometry (MS).
Journal ArticleDOI
How many human proteoforms are there
Ruedi Aebersold,Jeffrey N. Agar,I. Jonathan Amster,Mark S. Baker,Carolyn R. Bertozzi,Emily S. Boja,Catherine E. Costello,Benjamin F. Cravatt,Catherine Fenselau,Benjamin A. Garcia,Ying Ge,Jeremy Gunawardena,Ronald C. Hendrickson,Paul J. Hergenrother,Christian G. Huber,Alexander R. Ivanov,Ole N. Jensen,Michael C. Jewett,Neil L. Kelleher,Laura L. Kiessling,Nevan J. Krogan,Martin R. Larsen,Joseph A. Loo,Rachel R. Ogorzalek Loo,Emma Lundberg,Emma Lundberg,Michael J. MacCoss,Parag Mallick,Vamsi K. Mootha,Milan Mrksich,Tom W. Muir,Steven M. Patrie,James J. Pesavento,Sharon J. Pitteri,Henry Rodriguez,Alan Saghatelian,Wendy Sandoval,Hartmut Schlüter,Salvatore Sechi,Sarah A. Slavoff,Lloyd M. Smith,Michael Snyder,Paul M. Thomas,Mathias Uhlén,Jennifer E. Van Eyk,Marc Vidal,David R. Walt,Forest M. White,Evan R. Williams,Therese Wohlschlager,Vicki H. Wysocki,Nathan A. Yates,Nicolas L. Young,Bing Zhang +53 more
TL;DR: This work frames central issues regarding determination of protein-level variation and PTMs, including some paradoxes present in the field today, and uses this framework to assess existing data and ask the question, "How many distinct primary structures of proteins (proteoforms) are created from the 20,300 human genes?"
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Analytical strategies for phosphoproteomics
TL;DR: This review presents an overview of different analytical strategies for the characterization of phosphoproteins, focusing on the affinity methods utilized specifically for phosphoprotein and phosphopeptide enrichment prior to MS analysis, and on recent applications of these methods in cell biological applications.
Journal ArticleDOI
SIMAC (Sequential Elution from IMAC), a Phosphoproteomics Strategy for the Rapid Separation of Monophosphorylated from Multiply Phosphorylated Peptides
TL;DR: A simple and rapid strategy, SIMAC (sequential elution from IMAC), for sequential separation of monophosphorylation peptides and multiply phosphorylated peptides from highly complex biological samples is reported.