Christian A. Luber

TL;DR: A new intensity determination and normalization procedure called MaxLFQ is developed that is fully compatible with any peptide or protein separation prior to LC-MS analysis, which accurately detects the mixing ratio over the entire protein expression range, with greater precision for abundant proteins.

TL;DR: The SILAC-mouse approach is a versatile tool by which to quantitatively compare proteomes from knockout mice and thereby determine protein functions under complex in vivo conditions.

TL;DR: High-resolution mass spectrometry-based proteomics is used, combined with label-free quantitation algorithms, to determine the proteome of mouse splenic conventional and plasmacytoid DC subsets to a depth of 5,780 and 6,664 proteins, and found mutually exclusive expression of pattern recognition pathways not previously known to be different among conventional DC subset.

TL;DR: In humans and mice, CD8α+ conventional dendritic cells are the primary source of interferon-λ released in response to the adjuvant and Toll-like receptor 3 agonist poly IC.

TL;DR: High-resolution quantitative mass spectrometry is applied to directly and accurately compare the in vivo mouse mitochondrial proteomes of brown and white adipocytes to directly address functional questions in metabolism.