D
D.J. Benton
Researcher at Francis Crick Institute
Publications - 29
Citations - 2713
D.J. Benton is an academic researcher from Francis Crick Institute. The author has contributed to research in topics: Virus & Influenza A virus. The author has an hindex of 17, co-authored 26 publications receiving 1565 citations. Previous affiliations of D.J. Benton include National Institute for Medical Research.
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Journal ArticleDOI
Preexisting and de novo humoral immunity to SARS-CoV-2 in humans
Kevin W. Ng,Nikhil Faulkner,Georgina H. Cornish,Annachiara Rosa,Ruth Harvey,Saira Hussain,Rachel Ulferts,Christopher Earl,A.G. Wrobel,D.J. Benton,Chloe Roustan,William Bolland,Rachael Thompson,Ana Agua-Doce,Philip Hobson,Judith Heaney,Hannah Rickman,Stavroula Paraskevopoulou,Catherine F Houlihan,Kirsty Thomson,Emilie Sanchez,Gee Yen Shin,Moira J. Spyer,Dhira Joshi,Nicola O’Reilly,Philip A. Walker,Svend Kjaer,Andrew Riddell,Catherine Moore,Bethany R. Jebson,Meredyth G. Ll Wilkinson,Lucy R. Marshall,Elizabeth C. Rosser,Anna Radziszewska,Hannah Peckham,Coziana Ciurtin,Lucy R. Wedderburn,Rupert Beale,Charles Swanton,Sonia Gandhi,Brigitta Stockinger,John W. McCauley,Steve Gamblin,Laura E. McCoy,Peter Cherepanov,Eleni Nastouli,George Kassiotis,George Kassiotis +47 more
TL;DR: Using diverse assays for antibodies recognizing SARS-CoV-2 proteins, preexisting humoral immunity was detected and antibodies targeting the S2 subunit were particularly prevalent in children and adolescents.
Journal ArticleDOI
Receptor binding and priming of the spike protein of SARS-CoV-2 for membrane fusion.
D.J. Benton,A.G. Wrobel,Pengqi Xu,Pengqi Xu,Chloe Roustan,Stephen R. Martin,Peter B. Rosenthal,John J. Skehel,Steven J. Gamblin +8 more
TL;DR: Cryo-electron microscopy structures of consecutive binding events of ACE2 in complex with the spikeprotein of SARS-CoV-2 reveal the mechanisms of receptor binding by the spike protein and activation for membrane fusion by the Spike protein of Sars-Co V-2.
Journal ArticleDOI
SARS-CoV-2 and bat RaTG13 spike glycoprotein structures inform on virus evolution and furin-cleavage effects.
A.G. Wrobel,D.J. Benton,Pengqi Xu,Pengqi Xu,Chloe Roustan,Stephen R. Martin,Peter B. Rosenthal,John J. Skehel,Steven J. Gamblin +8 more
TL;DR: Cryo-EM and functional analyses of furin-cleaved spike from SARS-CoV-2 and the closely related spike from bat virus RaTG13 reveal differences in protein stability and binding to human receptor ACE2.
Journal ArticleDOI
The effect of the D614G substitution on the structure of the spike glycoprotein of SARS-CoV-2.
D.J. Benton,A.G. Wrobel,Chloe Roustan,Annabel Borg,Pengqi Xu,Stephen R. Martin,Peter B. Rosenthal,John J. Skehel,Steven J. Gamblin +8 more
TL;DR: In this paper, the authors used cryo-electron microscopy to compare G614 and D614 spikes and showed that the G614 mutant spike adopts a range of more open conformations that may facilitate binding to the SARS-CoV-2 receptor, ACE2, and subsequent structural rearrangements required for viral membrane fusion.
Posted ContentDOI
Pre-existing and de novo humoral immunity to SARS-CoV-2 in humans
Kevin W. Ng,Nikhil Faulkner,Georgina H. Cornish,Annachiara Rosa,Chris Earl,A.G. Wrobel,D.J. Benton,Chloe Roustan,William Bolland,Rachael Thompson,Ana Agua-Doce,Philip Hobson,Judith Heaney,Hannah Rickman,Stavroula Paraskevopoulou,Catherine F Houlihan,Kirsty Thomson,Emilie Sanchez,Gee Yen Shin,Moira J. Spyer,Philip A. Walker,Svend Kjaer,Andrew Riddell,Rupert Beale,Charles Swanton,Sonia Gandhi,Brigitta Stockinger,Steve Gamblin,Laura E. McCoy,Peter Cherepanov,Eleni Nastouli,George Kassiotis +31 more
TL;DR: The presence of pre-existing humoral immunity in uninfected and unexposed humans to the new coronavirus is demonstrated and identified as SARS-CoV-2 S-reactive antibodies, which were readily detectable by a sensitive flow cytometry-based method in SARS/COVID-19-uninfected individuals and were particularly prevalent in children and adolescents.