Elham Khatamzas

TL;DR: Over overlapping 15 amino acid peptide mixes may facilitate the analysis of antigen-specific CD4+ and CD8+ T-cell responses by cytokine flow cytometry, using clinical specimens that include shipped blood or cryopreserved PBMC.

TL;DR: Antigen-specific, cytokine flow cytometry was used to analyze the prevalence and frequency of CD4 and CD8 memory T cells specific for the abundantly expressed cytomegalovirus (CMV) phosphoprotein 65 in healthy CMV IgG-seropositive individuals and several new T cell epitopes were identified.

TL;DR: The results confirmed the previous contention that HCMV IE‐1 is an important CD8 T cell target and the technical improvement made in order to address this question has clearly wider implications.

TL;DR: A novel flow cytometric technique that allows simultaneous detection of human cytomegalovirus (HCMV)‐specific CD8pos.

TL;DR: The CMV-specific CD4+ T cell memory repertoire in normal subjects is characterized by striking clonotypic dominance and the potential for epitope focusing, suggesting that primary responsibility for immunosurveillance against CMV reactivation rests with a handful of clones recognizing a limited array of CMV determinants.