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Elisa D’Este
Researcher at Max Planck Society
Publications - 57
Citations - 3380
Elisa D’Este is an academic researcher from Max Planck Society. The author has contributed to research in topics: STED microscopy & Chemistry. The author has an hindex of 20, co-authored 47 publications receiving 2472 citations. Previous affiliations of Elisa D’Este include AREA Science Park.
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Journal ArticleDOI
Systematic Tuning of Rhodamine Spirocyclization for Super-resolution Microscopy.
Nicolas Lardon,Nicolas Lardon,Lu Wang,Lu Wang,Aline Tschanz,Philipp Hoess,Mai Tran,Elisa D’Este,Jonas Ries,Kai Johnsson,Kai Johnsson +10 more
TL;DR: This work describes here how the conversion of the ortho-carboxy moiety of a given rhodamine into substituted acyl benzenesulfonamides and alkylamides permits the systematic tuning of the equilibrium of spirocyclization with unprecedented accuracy and over a large range.
Journal ArticleDOI
Dual channel RESOLFT nanoscopy by using fluorescent state kinetics.
TL;DR: It is shown that RESOLFT fluorescence nanoscopy, a low light level scanning superresolution technique employing reversibly switchable fluorescent proteins (rsFPs), is capable of dual-channel live-cell imaging that is virtually free of chromatic errors and temporal offsets.
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Characterization of prion protein function by focal neurite stimulation
Ladan Amin,Xuan T. A. Nguyen,Irene Giulia Rolle,Elisa D’Este,Gabriele Giachin,Thanh Hoa Tran,Vladka Čurin Šerbec,Dan Cojoc,Giuseppe Legname +8 more
TL;DR: A function for the soluble form of PrP to promote fast neurite outgrowth and facilitate growth cone guidance is revealed by using a novel approach to stimulate and guide the growth cones of hippocampal neurons with high spatial and temporal resolution.
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Fluoreszierende Rhodamine und fluorogene Carbopyronine für die STED‐Mikroskopie lebender Zellen
Alexey N. Butkevich,Gyuzel Yu. Mitronova,Sven C. Sidenstein,Jessica L. Klocke,Dirk Kamin,Dirk N. H. Meineke,Elisa D’Este,Philip-Tobias Kraemer,Johann G. Danzl,Vladimir N. Belov,Stefan W. Hell +10 more
TL;DR: In this article, a farbstoff-Ligand-Konjugate-based farbung with Rhodamine and Carbopyronine with Absorptionsmaxima im Bereich von λ=500-630 µm is presented.
Journal ArticleDOI
Interrogating surface versus intracellular transmembrane receptor populations using cell-impermeable SNAP-tag substrates.
Pascal Poc,Vanessa A. Gutzeit,Julia Ast,Julia Ast,Joon Lee,Ben Jones,Elisa D’Este,Bettina Mathes,Martin Lehmann,David J. Hodson,David J. Hodson,Joshua Levitz,Johannes Broichhagen +12 more
TL;DR: A novel approach to improve extracellular labelling by functionalizing the SNAP-tag substrate benzyl guanine with a charged sulfonate with the utility of SBG-conjugated fluorophores to interrogate class A, B and C G protein-coupled receptors (GPCRs) using a range of imaging approaches.