Elisa D’Este

TL;DR: A general strategy to transform regular fluorophores into fluorogenic probes with an excellent cell permeability and a low unspecific background signal is reported, which was used for wash-free, multicolour, live-cell confocal and STED microscopy.

TL;DR: Glyoxal acted faster than PFA, cross‐linked proteins more effectively, and improved the preservation of cellular morphology, suggesting that glyoxal can be a valuable alternative to PFA for immunostaining.

TL;DR: A range of bright and photostable rhodamines and carbopyronines with absorption maxima in the range of λ=500–630 nm were prepared, and enabled the specific labeling of cytoskeletal filaments using HaloTag technology followed by staining with 1 μm solutions of the dye–ligand conjugates.

TL;DR: DyMIN (standing for Dynamic Intensity Minimum) scanning is introduced, generalizing and expanding on earlier concepts of RESCue and MINFIELD to reduce sample exposure and use as much on/off-switching light as needed to image at the desired resolution.

TL;DR: Cell-permeant red-emitting fluorescent dye labels with >140 nm Stokes shifts based on 9-iminoanthrone, 9-Imino-10-silaxanthone, and 9-IMino- 10-germaxanthone fluorophores demonstrate low toxicity and enable stimulated emission depletion (STED) nanoscopy in neurons, human fibroblasts, U2OS, and HeLa cells.