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Elizabeth A. Mendiaz
Researcher at Amgen
Publications - 19
Citations - 5737
Elizabeth A. Mendiaz is an academic researcher from Amgen. The author has contributed to research in topics: Stem cell factor & Amino acid. The author has an hindex of 16, co-authored 19 publications receiving 5520 citations.
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Journal ArticleDOI
Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE.
Robert Vassar,Brian D. Bennett,Safura Babu-Khan,Steve Kahn,Elizabeth A. Mendiaz,Paul Denis,David B. Teplow,Sandra Ross,Patricia Amarante,Richard Loeloff,Yi Luo,Seth Fisher,Janis Fuller,Steven P. Edenson,Jackson Lile,Mark A. Jarosinski,Anja Leona Biere,Eileen Curran,Teresa L. Burgess,Jean Claude Louis,Frank H. Collins,James J. S. Treanor,Gary Rogers,Martin Citron +23 more
TL;DR: Overexpression of a transmembrane aspartic protease, termed BACE (for beta-site APP-cleaving enzyme) increased the amount of beta-secretase cleavage products, and these were cleaved exactly and only at known beta- secretase positions.
Journal ArticleDOI
Identification, purification, and biological characterization of hematopoietic stem cell factor from buffalo rat liver-conditioned medium
Krisztina M. Zsebo,Jette Wypych,Ian K. McNiece,Hsieng Sen Lu,Kent A. Smith,Subash B. Karkare,Raj Kumar Sachdev,Victoria N. Yuschenkoff,Neal C. Birkett,Lee R. Williams,Vasuki Nagaraju Satyagal,Weifong Tung,Robert A. Bosselman,Elizabeth A. Mendiaz,Keith Langley +14 more
TL;DR: A novel growth factor, stem cell factor (SCF), for primitive hematopoietic progenitors based on its activity on bone marrow cells derived from mice treated with 5-fluorouracil is identified and sequences of partial SCF cDNAs are presented.
Journal ArticleDOI
Characterization of Alzheimer's beta -secretase protein BACE. A pepsin family member with unusual properties.
Mitsuru Haniu,Paul Denis,Yunjen Young,Elizabeth A. Mendiaz,Janis Fuller,John O. Hui,Brian D. Bennett,Steven Kahn,Sandra Ross,Teresa L. Burgess,Viswanatham Katta,Gary Rogers,Robert Vassar,Martin Citron +13 more
TL;DR: It is demonstrated that BACE is an N-glycosylated integral membrane protein that undergoes constitutive N-terminal processing in the Golgi apparatus and the presence of a transmembrane domain and the unusual disulfide bond structure lead us to conclude that Bace is an atypical pepsin family member.
Journal ArticleDOI
Structure of the active core of human stem cell factor and analysis of binding to its receptor kit.
Xuliang Jiang,Ogan Gurel,Elizabeth A. Mendiaz,George Stearns,Christi L. Clogston,Hsieng Sen Lu,Osslund Timothy D,Rashid Syed,Keith Langley,Wayne A. Hendrickson,Wayne A. Hendrickson +10 more
TL;DR: The crystal structure of selenomethionyl soluble human SCF is determined at 2.2 Å resolution by multiwavelength anomalous diffraction phasing and it is found that the SCF dimer has a symmetric ‘head‐to‐head’ association.
Journal ArticleDOI
Glycosylated and unglycosylated recombinant-derived human stem cell factors are dimeric and have extensive regular secondary structure.
Tsutomu Arakawa,D.A. Yphantis,J.W. Lary,Linda O. Narhi,Hsieng Sen Lu,S J Prestrelski,Christi L. Clogston,Krisztina M. Zsebo,Elizabeth A. Mendiaz,Jette Wypych +9 more
TL;DR: The structural characterization of soluble forms of recombinant human SCF purified from Escherichia coli (unglycosylated) and from Chinese hamster ovary (CHO) cells (glycosylate) is described.