E
Ellen M DeGennaro
Researcher at Massachusetts Institute of Technology
Publications - 13
Citations - 1818
Ellen M DeGennaro is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: CRISPR/Cpf1 & Trans-activating crRNA. The author has an hindex of 8, co-authored 11 publications receiving 1207 citations. Previous affiliations of Ellen M DeGennaro include Broad Institute & Howard Hughes Medical Institute.
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Journal ArticleDOI
Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array
Bernd Zetsche,Matthias Heidenreich,Prarthana Mohanraju,Iana Fedorova,Jeroen Kneppers,Jeroen Kneppers,Ellen M DeGennaro,Ellen M DeGennaro,Nerges Winblad,Sourav R Choudhury,Omar O. Abudayyeh,Jonathan S. Gootenberg,Wen Y Wu,David A. Scott,David A. Scott,Konstantin Severinov,Konstantin Severinov,Konstantin Severinov,John van der Oost,Feng Zhang +19 more
TL;DR: It is shown that the ability of Cpf1 to process its own CRISPR RNA (crRNA) can be used to simplify multiplexed genome editing.
Journal ArticleDOI
Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies
Paul W. Tillberg,Fei Chen,Kiryl D. Piatkevich,Yongxin Zhao,Chih-Chieh Yu,Brian P. English,Linyi Gao,Anthony J Martorell,Ho-Jun Suk,Fumiaki Yoshida,Fumiaki Yoshida,Ellen M DeGennaro,Douglas H. Roossien,Guanyu Gong,Uthpala Seneviratne,Steven R. Tannenbaum,Robert Desimone,Dawen Cai,Edward S. Boyden +18 more
TL;DR: ProExM as mentioned in this paper is a variant of ExM in which proteins are anchored to the swellable gel, allowing the use of conventional fluorescently labeled antibodies and streptavidin, and fluorescent proteins.
Journal ArticleDOI
A Source for Feature-Based Attention in the Prefrontal Cortex
TL;DR: The results suggest that VPA neurons compute the locations of objects with the features sought and send this information to FEF to guide eye movements to those relevant stimuli.
Posted ContentDOI
Multiplex gene editing by CRISPR-Cpf1 through autonomous processing of a single crRNA array
Bernd Zetsche,Matthias Heidenreich,Prarthana Mohanraju,Iana Fedorova,Jeroen Kneppers,Ellen M DeGennaro,Nerges Winblad,Sourav R Choudhury,Omar O. Abudayyeh,Jonathan S. Gootenberg,Wen Y Wu,David A. Scott,Konstantin Severinov,John van der Oost,Feng Zhang +14 more
TL;DR: This work shows for two Cpf1 orthologs that no other factors are required for array processing and demonstrates multiplex gene editing in mammalian cells as well as in the mouse brain by using a designed single CRISPR array.
Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies
Paul W. Tillberg,Fei Chen,Kiryl D. Piatkevich,Yongxin Zhao,Chih-Chieh Yu,Brian P. English,Linyi Gao,Anthony J Martorell,Ho-Jun Suk,Fumiaki Yoshida,Fumiaki Yoshida,Ellen M DeGennaro,Douglas H. Roossien,Guanyu Gong,Uthpala Seneviratne,Steven R. Tannenbaum,Robert Desimone,Dawen Cai,Edward S. Boyden +18 more
TL;DR: Protein retention ExM is described, a variant of ExM in which proteins are anchored to the swellable gel, allowing the use of conventional fluorescently labeled antibodies and streptavidin, and fluorescent proteins, for multicolor super-resolution imaging of cells and mammalian tissues on conventional microscopes.