Wen Y Wu

TL;DR: It is shown that the ability of Cpf1 to process its own CRISPR RNA (crRNA) can be used to simplify multiplexed genome editing.

TL;DR: This work shows for two Cpf1 orthologs that no other factors are required for array processing and demonstrates multiplex gene editing in mammalian cells as well as in the mouse brain by using a designed single CRISPR array.

TL;DR: Diversity, mechanisms and practical aspects of natural and engineered CRISPR-associated nucleases for genome engineering applications, including nuclease regulation and delivery, target specificity, as well as host repair diversity are explored.

TL;DR: In the version of this article initially published, the percentage for the targets Mecp2, Nlgn3, and Drd1 should be 15.2%, not 16.9%; the same error appeared in the main text, next to last paragraph, “The authors' results show that ∼17%.

TL;DR: This work established a sensitive and robust in vivo targeting assay based on loss of a target plasmid encoding the red fluorescent protein (mRFP), and suggests that folding of both the precursor guide (pre-cr RNA) and the mature guide (crRNA) have a major influence on Cas12a activity.