Haiyan Chen

TL;DR: The authors showed that immunization of macaques with nanoparticles conjugated with the receptor-binding domain of SARS-CoV-2, and adjuvanted with 3M-052 and alum, elicits cross-neutralizing antibody responses against bat coronaviruses, SARS, CoV and SARS CoV2.

TL;DR: This work uses a novel experimental workflow that includes an up-front complete or partial enzymatic deglycosylation step before trypsin digestion to characterize the gly cosylation patterns and maximize the glycosylations coverage of two recombinant HIV-1 transmitted/founder envelope oligomers derived from clade B and C viruses isolated from acute infection and expressed in 293T cells.

TL;DR: Exogenous membrane-anchored Envs, which can be produced in large quantities in mammalian cells, also display a virion-like glycan profile, where the glycoprotein is extensively decorated with high-mannose glycans, a carbohydrate profile that would be desirable to mimic with a vaccine.

TL;DR: This study elicited serum neutralizing HIV-1 antibodies in human bnAb precursor knock-in mice and wild-type macaques vaccinated with immunogens designed to select for improbable mutations, and shows that immunogens should exhibit differences in affinity across antibody maturation stages.

TL;DR: Analysis of A244 gp120, with or without Δ11 or gD, demonstrated that the Δ11 deletion was sufficient for enhanced antigenicity to gp120 C1 region, conformational V2, and V1/V2 gp120 conformational epitopes.