D
Daniel C. Douek
Researcher at National Institutes of Health
Publications - 422
Citations - 52079
Daniel C. Douek is an academic researcher from National Institutes of Health. The author has contributed to research in topics: T cell & Cytotoxic T cell. The author has an hindex of 113, co-authored 376 publications receiving 44694 citations. Previous affiliations of Daniel C. Douek include John Radcliffe Hospital & University of Texas Southwestern Medical Center.
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Journal ArticleDOI
CD4+ T Cell Depletion during all Stages of HIV Disease Occurs Predominantly in the Gastrointestinal Tract
Jason M. Brenchley,Timothy W. Schacker,Laura E. Ruff,David Price,Jodie H. Taylor,Gregory J. Beilman,Phuong L. Nguyen,Alexander Khoruts,Matthew Larson,Ashley T. Haase,Daniel C. Douek +10 more
TL;DR: The nature and extent of CD4+ T cell depletion in lymphoid tissue is defined and mechanisms of profound depletion of specific T cell subsets related to elimination of CCR5+ CD4- T cell targets and disruption of T cell homeostasis that accompanies chronic immune activation are pointed to.
Journal ArticleDOI
Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulation
Michael R. Betts,Jason M. Brenchley,David Price,Stephen C. De Rosa,Daniel C. Douek,Mario Roederer,Richard A. Koup +6 more
TL;DR: A novel technique to enumerate antigen-specific CD8+ T cells using a marker expressed on the cell surface following activation induced degranulation, a necessary precursor of cytolysis, and CD107-expressing CD8+, expressing cognate T cell receptors (TCR), is presented.
Journal ArticleDOI
A human memory T cell subset with stem cell-like properties
Luca Gattinoni,Enrico Lugli,Yun Ji,Zoltan Pos,Chrystal M. Paulos,Máire F. Quigley,Máire F. Quigley,Jorge R. Almeida,Emma Gostick,Zhiya Yu,Carmine Carpenito,Ena Wang,Daniel C. Douek,David Price,David Price,Carl H. June,Francesco M. Marincola,Mario Roederer,Nicholas P. Restifo +18 more
TL;DR: A long-lived human memory T cell population that has an enhanced capacity for self-renewal and a multipotent ability to derive central memory, effector memory and effector T cells is described.
Journal ArticleDOI
HIV reservoir size and persistence are driven by T cell survival and homeostatic proliferation
Nicolas Chomont,Mohamed El-Far,Petronela Ancuta,Lydie Trautmann,Francesco A. Procopio,Bader Yassine-Diab,Geneviève Boucher,Mohamed Rachid Boulassel,Georges Ghattas,Jason M. Brenchley,Timothy W. Schacker,Brenna J. Hill,Daniel C. Douek,Jean-Pierre Routy,Elias K. Haddad,Elias K. Haddad,Rafick Pierre Sekaly +16 more
TL;DR: The results suggest that viral eradication might be achieved through the combined use of strategic interventions targeting viral replication and drugs that interfere with the self renewal and persistence of proliferating memory T cells.
Journal ArticleDOI
Impaired T H 17 cell differentiation in subjects with autosomal dominant hyper-IgE syndrome
Joshua D. Milner,Jason M. Brenchley,Jason M. Brenchley,Arian Laurence,Alexandra F. Freeman,Brenna J. Hill,Kevin M. Elias,Kevin M. Elias,Yuka Kanno,Christine Spalding,Houda Elloumi,Michelle L. Paulson,Joie Davis,Amy P. Hsu,Ava I. Asher,John J. O'Shea,Steven M. Holland,William E. Paul,Daniel C. Douek +18 more
TL;DR: Ex vivo T cells from subjects with HIES failed to produce IL-17, but not IL-2, tumour-necrosis factor or interferon-γ, on mitogenic stimulation with staphylococcal enterotoxin B or on antigenic stimulation with Candida albicans or streptokinase, which is consistent with a crucial role for STAT3 signalling in the generation of TH17 cells.