Kwan-Ki Hwang

TL;DR: The polyclonality and low mutation frequency of these VH1 antibodies reveal fundamental differences in the regulation and maturation of these ADCC-mediating responses compared to VH2 bNAbs, which recently described for CD4-binding site broadly neutralizing antibodies.

TL;DR: The development of a VH1-46-derived antibody lineage that matured to neutralize 90% of HIV-1 isolates is defined with longitudinal sampling from time-of-infection to suggest a vaccination strategy for inducing both lineages.

TL;DR: Novel linear Ig heavy- and light-chain gene expression cassettes were designed to express Ig V(H) and V(L) genes isolated from sorted single B cells as IgG1 antibody without a cloning step to constitute a highly efficient strategy for rapid expression of Ig genes for high-throughput screening and analysis without cloning.

TL;DR: Many HIV- 1 envelope-reactive antibodies shortly after HIV-1 transmission may arise from crow-reactsive memory B cells previously stimulated by non-HIV-1 host or microbial antigens.

TL;DR: By studying the cooperation among multiple V3-glycan B cell lineages and their coevolution with autologous virus throughout 5 years of infection, key events are identified in the ontogeny of a V3 -glycan bnAb lineage.