Jacques Neefjes

TL;DR: The mechanisms regulating loading and intracellular trafficking of MHC II are discussed and a unique and complex series of reactions are discussed.

TL;DR: This study designs tetraspanin-based pH-sensitive optical reporters that detect MVB–PM fusion using live total internal reflection fluorescence and dynamic correlative light–electron microscopy and highlights the modulatory dynamics of MVB exocytosis that will help to increase the understanding of exosome physiology and identify druggable targets in exosomal pathologies.

TL;DR: The dynamics of endolysosomal organelles should no longer be studied in isolation, but in the context of the whole endomembrane system.

TL;DR: This work shows that the bulk of the translocated peptides are rapidly released from the ER by a mechanism that requires adenosine triphosphate (ATP) and that could not be inhibited by GTP gamma S, and suggests that peptides that are too long for binding to class I molecules in the ER can be trimmed further in theER lumen or transported back to the cytosol.

TL;DR: TAP binds and preferentially translocates peptides with a length suitable for binding to MHC class I molecules, but peptides that are considerably longer may also be substrates.