Jacques Neefjes

TL;DR: A diversifiable synthetic platform for SUMO‐based probes is developed by using a direct linear synthesis method, which permits N‐ and C‐terminal labelling to incorporate dyes and reactive warheads, respectively, and demonstrates the versatility and specificity of the synthetic SUMO platform for in vitro and in-vivo characterization of the SUMO protease family.

TL;DR: A conditionally immortalized dendritic cell line was established from bone marrow of mice transgenic for a thermolabile mutant of the SV40 large T antigen under the control of the class I Kb promoter and shows characteristic dendrites, an abundance of acidic vesicles and are highly active in endocytosis.

TL;DR: The dynamics of CD63 and CD82 interaction with MHC-II and its chaperone HLA-DM are measured using confocal FRET technology to visualize for the first time the protein dynamics of tetraspanin assemblies in MVB subdomains.

TL;DR: It is shown in mouse models of inflammatory disease that immunoproteasomes help prevent the accumulation of harmful protein aggregates.

TL;DR: A pipeline that uses the large sequence variety of proteasome-generated spliced peptides and identifies spliced epitope candidates, which carry the mutations and bind the predominant HLA-I alleles with high affinity is described.