Jin-Soo Kim

TL;DR: Three genes of Saccharomyces cerevisiae are identified that enhance the nuclear fusion defect of kar1-1 yeast during conjugation and Mutant kem 1 strains are hypersensitive to benomyl, lose chromosomes at a rate 10-20-fold higher than KEM+ strains, and lose viability upon nitrogen starvation.

TL;DR: A structure-based design strategy was used to construct a fusion protein, TBP/ZF, in which the three zinc fingers of Zif268 were linked to the COOH terminus of yeast TBP, which formed an extraordinarily stable complex when bound to the appropriate composite DNA site.

TL;DR: Results suggest that dimeric BS-RNase enters cells by adsorptive rather than receptor-mediated endocytosis and then evades cytosolic ribonuclease inhibitor so as to degrade cellular RNA.

TL;DR: It is found that the peptide containing the three zinc fingers of Zif268 could efficiently repress activated transcription when bound to a site near the TATA box (19-fold repression) or when Bound to a sites near the initiator element (18-fold repression).

TL;DR: This report uses a series of transient and stable transfection experiments to demonstrate that the expression of a target gene can be controlled by changing the in vivo concentration of designer zinc finger proteins in a dose-dependent manner and reports that designer zinc Finger proteins can access their binding sites integrated into the genome and function as potent transcription factors.