J
Joseph R. Klim
Researcher at Harvard University
Publications - 22
Citations - 1705
Joseph R. Klim is an academic researcher from Harvard University. The author has contributed to research in topics: Motor neuron & Amyotrophic lateral sclerosis. The author has an hindex of 12, co-authored 17 publications receiving 1283 citations. Previous affiliations of Joseph R. Klim include Wisconsin Alumni Research Foundation & Broad Institute.
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ALS-implicated protein TDP-43 sustains levels of STMN2, a mediator of motor neuron growth and repair
Joseph R. Klim,Luis A. Williams,Francesco Limone,Irune Guerra San Juan,Brandi N. Davis-Dusenbery,Daniel A. Mordes,Aaron Burberry,Michael J. Steinbaugh,Kanchana K. Gamage,Rory Kirchner,Rob Moccia,Seth H. Cassel,Kuchuan Chen,Brian J. Wainger,Brian J. Wainger,Clifford J. Woolf,Kevin Eggan +16 more
TL;DR: In this paper, the authors report transcripts whose abundances in human motor neurons are sensitive to TDP-43 depletion, and they propose that restoring STMN2 expression warrants examination as a therapeutic strategy for ALS.
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A defined glycosaminoglycan-binding substratum for human pluripotent stem cells
TL;DR: The results indicate that synthetic substrates that recognize cell-surface glycans can facilitate the long-term culture of pluripotent stem cells.
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Modeling ALS with motor neurons derived from human induced pluripotent stem cells.
Samuel Sances,Lucie Bruijn,Siddharthan Chandran,Kevin Eggan,Ritchie Ho,Joseph R. Klim,Matthew R. Livesey,Emily Rhodes Lowry,Jeffrey D. Macklis,David Rushton,Cameron Sadegh,Dhruv Sareen,Hynek Wichterle,Su-Chun Zhang,Clive N. Svendsen +14 more
TL;DR: Common developmental principles of both lower and upper motor neuron development that have led to specific derivation techniques are discussed and how these motor neurons may be matured further either through direct expression or administration of specific factors or coculture approaches with other tissues are suggested.
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Genetic Ablation of AXL Does Not Protect Human Neural Progenitor Cells and Cerebral Organoids from Zika Virus Infection
Michael F. Wells,Michael F. Wells,Max R. Salick,Ole Wiskow,Daniel J. Ho,Kathleen A. Worringer,Robert J. Ihry,Sravya Kommineni,Bilada Bilican,Joseph R. Klim,Ellen J. Hill,Liam T. Kane,Chaoyang Ye,Ajamete Kaykas,Kevin Eggan,Kevin Eggan +15 more
TL;DR: It is shown that genetic ablation of AXL has no effect on ZikV entry or ZIKV-mediated cell death in human induced pluripotent stem cell (iPSC)-derived NPCs or cerebral organoids, calling into question the utility of A XL inhibitors for preventing birth defects after infection.
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How to make spinal motor neurons
Brandi N. Davis-Dusenbery,Brandi N. Davis-Dusenbery,Luis A. Williams,Joseph R. Klim,Joseph R. Klim,Kevin Eggan,Kevin Eggan,Kevin Eggan +7 more
TL;DR: This Primer discusses how the logic of spinal motor neuron development has been applied to allow generation of motor neurons either from pluripotent stem cells by directed differentiation and transcriptional programming, or from somatic cells by direct lineage conversion.