J
Joshua H. Wu
Researcher at University of Michigan
Publications - 16
Citations - 307
Joshua H. Wu is an academic researcher from University of Michigan. The author has contributed to research in topics: Biology & Stem cell. The author has an hindex of 6, co-authored 9 publications receiving 92 citations.
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Journal ArticleDOI
Mapping Development of the Human Intestinal Niche at Single-Cell Resolution.
Emily M. Holloway,Michael Czerwinski,Yu-Hwai Tsai,Joshua H. Wu,Angeline Wu,Charlie Childs,Katherine D. Walton,Caden W. Sweet,Qianhui Yu,Ian A. Glass,Barbara Treutlein,J. Gray Camp,Jason R. Spence +12 more
TL;DR: This work uses single-cell mRNA sequencing with in situ validation approaches to interrogate human intestinal development from 7-21 weeks post conception, assigning molecular identities and spatial locations to cells and factors that comprise the niche.
Journal ArticleDOI
Differentiation of Human Intestinal Organoids with Endogenous Vascular Endothelial Cells
Emily M. Holloway,Joshua H. Wu,Michael Czerwinski,Caden W. Sweet,Angeline Wu,Yu-Hwai Tsai,Sha Huang,Amy E. Stoddard,Meghan M. Capeling,Ian A. Glass,Jason R. Spence +10 more
TL;DR: HIOs can co-differentiate a native EC population that is properly patterned with an intestine-specific EC transcriptional signature in vitro, and it is found that HIO ECs grown in vitro share the highest similarity with native intestinal ECs relative to kidney and lung.
Journal ArticleDOI
Charting human development using a multi-endodermal organ atlas and organoid models
Qianhui Yu,Umut Kilik,Emily M. Holloway,Yu-Hwai Tsai,Christoph Harmel,Angeline Wu,Joshua H. Wu,Michael Czerwinski,Charlie Childs,Zhisong He,Meghan M. Capeling,Sha Huang,Ian A. Glass,Peter D.R. Higgins,Barbara Treutlein,Jason R. Spence,J. Gray Camp +16 more
TL;DR: This work generates a single-cell transcriptome atlas from multiple developing endodermal organs of the respiratory and gastrointestinal tract and implements the atlas as a high-dimensional search space to benchmark human pluripotent stem cell (hPSC)-derived intestinal organoids (HIOs) under multiple culture conditions.
Posted ContentDOI
Differentiation of human intestinal organoids with endogenous vascular endothelial cells
Emily M. Holloway,Joshua H. Wu,Michael Czerwinkski,Caden W. Sweet,Angeline Wu,Yu-Hwai Tsai,Sha Huang,Amy E. Stoddard,Meghan M. Capeling,Ian A. Glass,Jason R. Spence +10 more
TL;DR: HIOs can co-differentiate a native EC population that are properly patterned with an intestine-specific EC transcriptional signature in vitro, and are shown to share the highest similarity with native intestinal ECs relative to kidney and lung.
Posted ContentDOI
R-SPONDIN2+ Mesenchymal Cells Form the Bud Tip Progenitor Niche During Human Lung Development
Renee F.C. Hein,Joshua H. Wu,Yu-Hwai Tsai,Angeline Wu,Alyssa J. Miller,Emily M. Holloway,Tristan Frum,Ansley S. Conchola,Emmanuelle Szenker-Ravi,Bruno Reversade,Kelley S. Yan,Calvin J. Kuo,Jason R. Spence +12 more
TL;DR: In this article, single cell RNA sequencing data from multiple human lung specimens and identified a mesenchymal cell population present during development that is highly enriched for expression of the WNT agonist R-SPONDIN2 (RSPO2), and adjacent epithelial bud tip progenitors are enriched for the RSPO 2 receptor LGR5.