Joshua H. Wu

TL;DR: This work uses single-cell mRNA sequencing with in situ validation approaches to interrogate human intestinal development from 7-21 weeks post conception, assigning molecular identities and spatial locations to cells and factors that comprise the niche.

TL;DR: HIOs can co-differentiate a native EC population that is properly patterned with an intestine-specific EC transcriptional signature in vitro, and it is found that HIO ECs grown in vitro share the highest similarity with native intestinal ECs relative to kidney and lung.

TL;DR: This work generates a single-cell transcriptome atlas from multiple developing endodermal organs of the respiratory and gastrointestinal tract and implements the atlas as a high-dimensional search space to benchmark human pluripotent stem cell (hPSC)-derived intestinal organoids (HIOs) under multiple culture conditions.

TL;DR: HIOs can co-differentiate a native EC population that are properly patterned with an intestine-specific EC transcriptional signature in vitro, and are shown to share the highest similarity with native intestinal ECs relative to kidney and lung.

TL;DR: In this article, single cell RNA sequencing data from multiple human lung specimens and identified a mesenchymal cell population present during development that is highly enriched for expression of the WNT agonist R-SPONDIN2 (RSPO2), and adjacent epithelial bud tip progenitors are enriched for the RSPO 2 receptor LGR5.