Keith Walcott

TL;DR: This work reports the development of an amplification-free CRISPR-Cas13a assay for direct detection of SARS-CoV-2 from nasal swab RNA that can be read with a mobile phone microscope and has the potential to enable rapid, low-cost, point-of-care screening for Sars-Cov-2.

TL;DR: Pharmacological inhibition of phosphatidylinositol kinases and cholesterol homeostasis reduced replication of all three coronaviruses and offer important insights for the understanding of the coronavirus life cycle and the development of host-directed therapies.

TL;DR: In this article , a plasmid-based genome assembly and rescue strategy (pGLUE) was proposed to construct complete infectious viruses or noninfectious subgenomic replicons in a single ligation reaction with >80% efficiency.

TL;DR: In this paper , a plasmid-based genome assembly and rescue strategy (pGLUE) was proposed to construct complete infectious viruses or noninfectious subgenomic replicons in a single ligation reaction with >80% efficiency.

TL;DR: In this paper , the authors adapted a CRISPR/Cas9 system to facilitate targeted gene disruption in Histoplasma with high efficiency, and used this system to disrupt a number of target genes including RYP2 and SRE1 where loss-of-function mutations could be monitored visually by colony morphology or color.