Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy.
Parinaz Fozouni,Sungmin Son,María Díaz de León Derby,Gavin J. Knott,Gavin J. Knott,Carley N. Gray,Carley N. Gray,Michael V. D’Ambrosio,Chunyu Zhao,Neil A. Switz,G. Renuka Kumar,G. Renuka Kumar,Stephanie I. Stephens,Stephanie I. Stephens,Daniela Boehm,Daniela Boehm,Chia-Lin Tsou,Chia-Lin Tsou,Jeffrey Shu,Jeffrey Shu,Abdul Bhuiya,Maxim Armstrong,Andrew R. Harris,Pei Yi Chen,Pei Yi Chen,Jeannette M. Osterloh,Anke Meyer-Franke,Bastian Joehnk,Keith Walcott,Anita Sil,Charles Langelier,Katherine S. Pollard,Emily D. Crawford,Andreas S. Puschnik,Maira Phelps,Amy Kistler,Joseph L. DeRisi,Jennifer A. Doudna,Daniel A. Fletcher,Melanie Ott +39 more
TLDR
This work reports the development of an amplification-free CRISPR-Cas13a assay for direct detection of SARS-CoV-2 from nasal swab RNA that can be read with a mobile phone microscope and has the potential to enable rapid, low-cost, point-of-care screening for Sars-Cov-2.About:
This article is published in Cell.The article was published on 2021-01-21 and is currently open access. It has received 501 citations till now. The article focuses on the topics: Respiratory virus.read more
Citations
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Journal ArticleDOI
CRISPR-based diagnostics.
Michael M. Kaminski,Michael M. Kaminski,Omar O. Abudayyeh,Jonathan S. Gootenberg,Feng Zhang,James J. Collins +5 more
TL;DR: A review of the rapidly expanding toolbox for CRISPR-based diagnostics, in particular the various assays, preamplification strategies and readouts, and highlight their main applications in the sensing of a wide range of molecular targets relevant to human health is provided in this article.
Journal ArticleDOI
CRISPR technology incorporating amplification strategies: molecular assays for nucleic acids, proteins, and small molecules
Wei Feng,Ashley M. Newbigging,Jeffrey Tao,Yiren Cao,Hanyong Peng,Connie Le,Jinjun Wu,Bo Pang,Bo Pang,Juan Li,D. Lorne Tyrrell,Hongquan Zhang,X. Chris Le +12 more
TL;DR: Successful integrations of CRISPR technology with nucleic acid amplification techniques result in highly sensitive and rapid detection of SARS-CoV-2, the virus that causes the COVID-19 pandemic.
Journal ArticleDOI
Detection of SARS-CoV-2 and Its Mutated Variants via CRISPR-Cas13-Based Transcription Amplification.
Yuxi Wang,Yong Zhang,Junbo Chen,Minjin Wang,Ting Zhang,Wenxin Luo,Yalun Li,Yangping Wu,Bo Zeng,Kaixiang Zhang,Ruijie Deng,Weimin Li +11 more
TL;DR: In this paper, a light-up CRISPR-Cas13 transcription amplification method was used to detect SARS-CoV-2 and its mutated variants, and the proposed RNA virus assays are promising for point-of-care monitoring of SARS and its risking variants.
Journal ArticleDOI
An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing.
Kean Hean Ooi,Kean Hean Ooi,Mengying Mandy Liu,Mengying Mandy Liu,Jie Wen Douglas Tay,Jie Wen Douglas Tay,Seok Yee Teo,Seok Yee Teo,Pornchai Kaewsapsak,Shengyang Jin,Chun Kiat Lee,Jingwen Hou,Sebastian Maurer-Stroh,Weisi Lin,Benedict Yan,Gabriel Yan,Yong-Gui Gao,Meng How Tan,Meng How Tan +18 more
TL;DR: In this paper, a CRISPR-based diagnostic test for SARS-CoV-2 was proposed, which can be applied directly on nasopharyngeal (NP) specimens without RNA purification, and incorporates a human internal control within the same reaction.
Journal ArticleDOI
Accelerated RNA detection using tandem CRISPR nucleases.
Tina Y. Liu,Gavin J. Knott,Gavin J. Knott,Dylan C. J. Smock,John J Desmarais,Sungmin Son,Abdul Bhuiya,Shrutee Jakhanwal,Noam Prywes,Shreeya Agrawal,María Díaz de León Derby,Neil A. Switz,Maxim Armstrong,Andrew R. Harris,Emeric J Charles,Brittney W. Thornton,Parinaz Fozouni,Jeffrey Shu,Jeffrey Shu,Stephanie I. Stephens,Stephanie I. Stephens,G. Renuka Kumar,G. Renuka Kumar,Chunyu Zhao,Amanda Mok,Anthony T. Iavarone,Arturo M. Escajeda,Roger McIntosh,Shineui Kim,Eli J Dugan,Katherine S. Pollard,Katherine S. Pollard,Ming X. Tan,Melanie Ott,Melanie Ott,Daniel A. Fletcher,Liana F. Lareau,Patrick D. Hsu,David F. Savage,Jennifer A. Doudna +39 more
TL;DR: Fast Integrated Nuclease Detection In Tandem (FIND-IT) as mentioned in this paper combines RNA-guided Cas13 and Csm6 with a chemically stabilized activator to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA extracted from respiratory swab samples with quantitative reverse transcriptase PCR (qRT-PCR)-derived cycle threshold values up to 33.
References
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