Lorigail Echipare

TL;DR: This work combined MeDIP-seq with methylation-sensitive restriction enzyme (MRE-seq) sequencing for comprehensive methylome coverage at lower cost and detected regions with allele-specific epigenetic states, identifying most known imprinted regions and new loci with Monoallelic epigenetic marks and monoallelic expression.

TL;DR: This chapter describes in detail the steps involved in performing ChIP assays either manually or using the IP-Star ChIP robot, followed by a detailed protocol to prepare successful libraries for Illumina sequencing, point the reader to several methods by which massive ChIP-seq data sets can be analyzed to extract the tremendous information contained within.

TL;DR: It is suggested that the H3K9me3 at ZNF 3′ exons may function to protect the genome from inappropriate recombination rather than to regulate transcription.

TL;DR: A striking asymmetry in epigenomic reprogramming between luminal and myoepithelial cell types is found, with the genomes of luminal cells harbouring more than twice the number of hypomethylated enhancer elements compared with myoEPithelial cells.

TL;DR: The first genome-wide identification and characterization of TR4 in vivo binding and the identification of cell type specific TR4 binding sites enables future studies of the pathways underlying TR4 action and its possible role in metabolic diseases.