Phyllis A. Dennery

TL;DR: A critical analysis of the challenges and limitations of the most widely used fluorescent probes for detecting and measuring reactive oxygen and nitrogen species and proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results are presented.

TL;DR: It is concluded that gene regulation, DNA delivery and expression can now be noninvasively monitored in living mammals using a luciferase reporter, and real‐time, noninvasive study of gene expression in living animal models for human development and disease is possible.

TL;DR: There is a beneficial threshold of HO‐1 overexpression related to the accumulation of reactive iron released in the degradation of heme, which makes it unlikely that exaggerated expression ofHO‐1 is a cytoprotective response.

TL;DR: A better understanding of ROS-mediated reactions and their impact on embryonic development is important to ensure optimal outcomes.

TL;DR: It is speculated that nuclear localization of HO-1 protein may serve to up-regulate genes that promote cytoprotection against oxidative stress after exposure to hypoxia and heme or heme/hemopexin.