S
Serge Muyldermans
Researcher at Vrije Universiteit Brussel
Publications - 323
Citations - 30516
Serge Muyldermans is an academic researcher from Vrije Universiteit Brussel. The author has contributed to research in topics: Single-domain antibody & Antibody. The author has an hindex of 80, co-authored 305 publications receiving 26561 citations. Previous affiliations of Serge Muyldermans include Dalian University of Technology & Université libre de Bruxelles.
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Journal ArticleDOI
Modulation of protein properties in living cells using nanobodies
Axel Kirchhofer,Jonas Helma,Jonas Helma,Katrin Schmidthals,Katrin Schmidthals,Carina Frauer,Carina Frauer,Sheng Cui,Sheng Cui,Annette Karcher,Annette Karcher,Mireille Pellis,Serge Muyldermans,Corella S. Casas-Delucchi,M. Cristina Cardoso,Heinrich Leonhardt,Heinrich Leonhardt,Karl-Peter Hopfner,Karl-Peter Hopfner,Ulrich Rothbauer +19 more
TL;DR: It is shown that protein conformations can be manipulated and studied with nanobodies in living cells, and camelid-derived single-domain antibodies that modulate the conformation and spectral properties of the green fluorescent protein (GFP) are selected.
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Sequence and structure of VH domain from naturally occurring camel heavy chain immunoglobulins lacking light chains
TL;DR: The camel hypervariable regions might adopt structures which differ substantially from the known canonical structures, thereby increasing the repertoire of the camel antigen binding sites within a VH, as in the DAW antibody.
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Potent enzyme inhibitors derived from dromedary heavy‐chain antibodies
Marc Lauwereys,Mehdi Arbabi Ghahroudi,Aline Desmyter,Jörg Kinne,Wolfgang Hölzer,Erwin De Genst,Lode Wyns,Serge Muyldermans +7 more
TL;DR: Evidence is provided that dromedary heavy‐chain antibodies, in vivo‐matured in the absence of light chains, are a unique source of inhibitory antibodies that appear to be potent enzyme inhibitors when tested in chromogenic assays.
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General Strategy to Humanize a Camelid Single-domain Antibody and Identification of a Universal Humanized Nanobody Scaffold
Cécile Vincke,Remy Loris,Dirk Saerens,Dirk Saerens,Sergio Martínez-Rodríguez,Serge Muyldermans,Serge Muyldermans,Katja Conrath,Katja Conrath +8 more
TL;DR: In this article, the authors performed a humanization of Nanobodies of the subfamily of dromedary-derived antibodies and investigated the effects on antigen affinity, solubility, expression yield, and stability.
Journal ArticleDOI
Camelid immunoglobulins and nanobody technology.
Serge Muyldermans,Toya Nath Baral,V. Cortez Retamozzo,P. De Baetselier,E. De Genst,J. Kinne,Heinrich Leonhardt,Heinrich Leonhardt,Stefan Magez,Viet Nguyen,Hilde Revets,Ulrich Rothbauer,Ulrich Rothbauer,Benoit Stijlemans,Sergei Tillib,Ulrich Wernery,Lode Wyns,Gh. Hassanzadeh-Ghassabeh,Dirk Saerens +18 more
TL;DR: This work designed a strategy to clone the Nb repertoire of an immunized dromedary and to select the Nbs with specificity for the authors' target antigens, which are well produced in bacteria, are very stable and highly soluble, and bind their cognate antigen with high affinity and specificity.