Thomas Hoffmann

TL;DR: It is found that BRD4 acts as general coactivator of RNA polymerase II–dependent transcription, which is broadly repressed upon high-dose BETi treatment, and to interrogate direct responses to BET bromodomain inhibitors (BETis).

TL;DR: In this article, the authors adapted pooled RNA interference (RNAi) screening technology for use in orthotopic patient-derived xenograft models, creating a high-throughput negative-selection screening platform in a functional in vivo tumour microenvironment.

TL;DR: It is demonstrated that STAT3 plays an unexpected tumour-suppressive role in KRAS mutant lung adenocarcinoma (AC) by sequestering NF-κB within the cytoplasm, thereby inhibiting IL-8-mediated myeloid tumour infiltration and tumour vascularization and hence tumour progression.

TL;DR: The power of in vivo phenotypic screening to identify new classes of ‘cancer dependencies’ not identified by previous in vitro approaches is demonstrated, and could supply new opportunities for therapeutic intervention.

TL;DR: SplashRNA is presented, a sequential classifier to predict potent microRNA-based short hairpin RNAs (shRNAs) and outperforms previous algorithms and reliably predicts the most efficient shRNAs for a given gene.