T
Toni Pfaffeneder
Researcher at ETH Zurich
Publications - 29
Citations - 2702
Toni Pfaffeneder is an academic researcher from ETH Zurich. The author has contributed to research in topics: 5-Hydroxymethylcytosine & Oligonucleotide. The author has an hindex of 17, co-authored 28 publications receiving 2496 citations. Previous affiliations of Toni Pfaffeneder include Imperial College London & Ludwig Maximilian University of Munich.
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Journal ArticleDOI
Dynamic Readers for 5-(Hydroxy)Methylcytosine and Its Oxidized Derivatives
Cornelia G. Spruijt,Felix Gnerlich,Arne H. Smits,Toni Pfaffeneder,Pascal W.T.C. Jansen,Christina Bauer,Martin Münzel,Mirko Wagner,Markus Müller,Fariha Khan,Fariha Khan,H. Christian Eberl,Anneloes Mensinga,Arie B. Brinkman,Konstantin Lephikov,Udo Müller,Jörn Walter,Rolf Boelens,Hugo van Ingen,Heinrich Leonhardt,Thomas Carell,Michiel Vermeulen +21 more
TL;DR: Oxidized derivatives of mC recruit distinct transcription regulators as well as a large number of DNA repair proteins in mouse ES cells, implicating the DNA damage response as a major player in active DNA demethylation.
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The Discovery of 5-Formylcytosine in Embryonic Stem Cell DNA†
Toni Pfaffeneder,Benjamin Hackner,Matthias Truß,Martin Münzel,Markus Müller,Christian A. Deiml,Christian Hagemeier,Thomas Carell +7 more
TL;DR: Recent data suggest that active demethylation in postdevelopmental phases may proceed through deamination of hmC to give 5-hydroxymethyluridine (hmU), which is then removed from the genome with the help of the base excision repair (BER) system.
Journal ArticleDOI
Tet oxidizes thymine to 5-hydroxymethyluracil in mouse embryonic stem cell DNA
Toni Pfaffeneder,Fabio Spada,Mirko Wagner,Caterina Brandmayr,Silvia K. Laube,David Eisen,Matthias Truss,Jessica Steinbacher,Benjamin Hackner,Olga Kotljarova,David Schuermann,Stylianos Michalakis,Olesea Kosmatchev,Stefan Schiesser,Barbara Steigenberger,Nada Raddaoui,Gengo Kashiwazaki,Udo Müller,Cornelia G. Spruijt,Michiel Vermeulen,Heinrich Leonhardt,Primo Schär,Markus Müller,Thomas Carell +23 more
TL;DR: Protein pull-down experiments in combination with peptide tracing identifies hmU as a base that influences binding of chromatin remodeling proteins and transcription factors, suggesting that hm U has a specific function in stem cells besides triggering DNA repair.
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Low values of 5-hydroxymethylcytosine (5hmC), the "sixth base," are associated with anaplasia in human brain tumors.
Theo F. J. Kraus,Daniel Globisch,Mirko Wagner,Sabina Eigenbrod,David Widmann,Martin Münzel,Markus Müller,Toni Pfaffeneder,Benjamin Hackner,Wolfgang Feiden,Ulrich Schüller,Thomas Carell,Hans A. Kretzschmar +12 more
TL;DR: The number of 5hmC positive cells and the amount of 5mC/dG in the genome that has been proposed to be related to pluripotency and lineage commitment in embryonic stem cells is also associated with brain tumor differentiation and anaplasia.
Journal ArticleDOI
Mechanism and stem-cell activity of 5-carboxycytosine decarboxylation determined by isotope tracing.
Stefan Schiesser,Benjamin Hackner,Toni Pfaffeneder,Markus Müller,Christian Hagemeier,Matthias Truss,Thomas Carell +6 more
TL;DR: Stem cells seem to erase epigenetic information by decarboxylation of the newly discovered epigenetic base 5-carboxycytosine (caC), which is likely to involve a nucleophilic attack of the C5-C6 double bond.