Y
Yu-Hwai Tsai
Researcher at University of Michigan
Publications - 34
Citations - 1408
Yu-Hwai Tsai is an academic researcher from University of Michigan. The author has contributed to research in topics: Induced pluripotent stem cell & Stem cell. The author has an hindex of 10, co-authored 34 publications receiving 878 citations.
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Journal ArticleDOI
In vitro generation of human pluripotent stem cell derived lung organoids
Briana R. Dye,David R. Hill,Michael A H Ferguson,Yu-Hwai Tsai,Melinda S. Nagy,Rachel Dyal,James M. Wells,Christopher N. Mayhew,Roy Nattiv,Ophir D. Klein,Eric S. White,Gail H. Deutsch,Jason R. Spence +12 more
TL;DR: It is shown that HLOs are remarkably similar to human fetal lung based on global transcriptional profiles, suggesting that HL Os are an excellent model to study human lung development, maturation and disease.
Journal ArticleDOI
Nonadhesive Alginate Hydrogels Support Growth of Pluripotent Stem Cell-Derived Intestinal Organoids.
Meghan M. Capeling,Michael Czerwinski,Sha Huang,Yu-Hwai Tsai,Angeline Wu,Melinda S. Nagy,Benjamin A. Juliar,Nambirajan Sundaram,Yang Song,Woojin M. Han,Shuichi Takayama,Eben Alsberg,Andrés J. García,Michael A. Helmrath,Andrew J. Putnam,Jason R. Spence +15 more
TL;DR: Alginate, a minimally supportive hydrogel with no inherent cell instructive properties, supports HIO growth in vitro and leads to HIO epithelial differentiation that is virtually indistinguishable from Matrigel-grown HIOs.
Journal ArticleDOI
In Vitro and In Vivo Development of the Human Airway at Single-Cell Resolution.
Alyssa J. Miller,Qianhui Yu,Qianhui Yu,Michael Czerwinski,Yu-Hwai Tsai,Renee F. Conway,Angeline Wu,Emily M. Holloway,Taylor Walker,Ian A. Glass,Barbara Treutlein,Barbara Treutlein,J. Gray Camp,J. Gray Camp,Jason R. Spence +14 more
TL;DR: This work used homogeneous human bud tip organoid cultures and identified SMAD signaling as a key regulator of the bud tip-to-airway transition and shed light on human airway differentiation in vitro and provides a single-cell atlas of the developing human lung.
Journal ArticleDOI
Mapping Development of the Human Intestinal Niche at Single-Cell Resolution.
Emily M. Holloway,Michael Czerwinski,Yu-Hwai Tsai,Joshua H. Wu,Angeline Wu,Charlie Childs,Katherine D. Walton,Caden W. Sweet,Qianhui Yu,Ian A. Glass,Barbara Treutlein,J. Gray Camp,Jason R. Spence +12 more
TL;DR: This work uses single-cell mRNA sequencing with in situ validation approaches to interrogate human intestinal development from 7-21 weeks post conception, assigning molecular identities and spatial locations to cells and factors that comprise the niche.
Journal ArticleDOI
Differentiation of Human Intestinal Organoids with Endogenous Vascular Endothelial Cells
Emily M. Holloway,Joshua H. Wu,Michael Czerwinski,Caden W. Sweet,Angeline Wu,Yu-Hwai Tsai,Sha Huang,Amy E. Stoddard,Meghan M. Capeling,Ian A. Glass,Jason R. Spence +10 more
TL;DR: HIOs can co-differentiate a native EC population that is properly patterned with an intestine-specific EC transcriptional signature in vitro, and it is found that HIO ECs grown in vitro share the highest similarity with native intestinal ECs relative to kidney and lung.