Yu-Hwai Tsai

TL;DR: It is shown that HLOs are remarkably similar to human fetal lung based on global transcriptional profiles, suggesting that HL Os are an excellent model to study human lung development, maturation and disease.

TL;DR: Alginate, a minimally supportive hydrogel with no inherent cell instructive properties, supports HIO growth in vitro and leads to HIO epithelial differentiation that is virtually indistinguishable from Matrigel-grown HIOs.

TL;DR: This work used homogeneous human bud tip organoid cultures and identified SMAD signaling as a key regulator of the bud tip-to-airway transition and shed light on human airway differentiation in vitro and provides a single-cell atlas of the developing human lung.

TL;DR: This work uses single-cell mRNA sequencing with in situ validation approaches to interrogate human intestinal development from 7-21 weeks post conception, assigning molecular identities and spatial locations to cells and factors that comprise the niche.

TL;DR: HIOs can co-differentiate a native EC population that is properly patterned with an intestine-specific EC transcriptional signature in vitro, and it is found that HIO ECs grown in vitro share the highest similarity with native intestinal ECs relative to kidney and lung.