Zhuhao Wu

TL;DR: iDISCO enables facile volume imaging of immunolabeled structures in complex tissues and reveals unexpected variability in number of apoptotic neurons within individual sensory ganglia despite tight control of total number in all ganglia.

TL;DR: A pipeline for high-speed acquisition of brain activity at cellular resolution through profiling immediate early gene expression using immunostaining and light-sheet fluorescence imaging, followed by automated mapping and analysis of activity by an open-source software program that is widely applicable to different experimental paradigms, including animal species for which transgenic activity reporters are not readily available.

TL;DR: A robust and efficient platform for imaging and reconstructing complete neuronal morphologies, including axonal arbors that span substantial portions of the brain are presented and axonal shapes revealed previously unknown subtypes of projection neurons and suggest organizational principles of long-range connectivity.

TL;DR: Using traumatic injury as a model, this work systematically investigates mitogen-activated protein kinase (MAPK) families and delineates a MAPK cascade that represents the early degenerative response to axonal injury, revealing a regulatory mechanism that integrates distinct signals to instruct pathological axon degeneration.

TL;DR: There is strong evidence that during action potential (AP) firing, nerve terminals rely on the glucose transporter GLUT4 as a glycolytic regulatory system to meet the activity-driven increase in energy demands.